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Product Info Summary
| SKU: | PROTP0AC53 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
e.coli G6PD Recombinant Protein
SKU/Catalog Number
PROTP0AC53
Size
Starting from 20ug
Tag
Non-Tagged
Description
E. coli G6PD Recombinant Protein expressed in E. coli (non-tagged). Sequence domain: 1-491aa. Application(s): Enzyme Activity, SDS-PAGE.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Cite This Product
e.coli G6PD Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP0AC53)
Form
Liquid
Formulation
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
55.7 kDa (491aa) confirmed by MALDI-TOF
Amino Acid Sequence
E.coli, P0AC53, 1-491aa; MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF EBioactivity
Specific activity is > 50 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For e.coli G6PD Recombinant Protein (PROTP0AC53)
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