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Product Info Summary
| SKU: | PROTP61889 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
e.coli mdh Recombinant Protein
View all mdh recombinant proteins
SKU/Catalog Number
PROTP61889
Size
Starting from 20ug
Tag
His-Tag
Description
E. coli mdh Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-312aa. Application(s): Enzyme Activity, SDS-PAGE.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
e.coli mdh Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP61889)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Amino Acid Sequence
E.coli, P61889, 1-312aa; MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNKBioactivity
Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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