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Product Info Summary
| SKU: | EKC1753 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
ELL Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1753
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The ELL Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ELL protein expression profile in cells. The kit can be used for measuring the relative amounts of ELL in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ELL.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ELL Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1753)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1753 is reactive to ELL in Human, Mouse samples
Application Guarantee
EKC1753 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-ELL Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from Jurkat/HeLa cells
Protein Target Info & Infographic
Gene/Protein Information For ELL (Source: Uniprot.Org, NCBI)
Gene Name
ELL
Full Name
RNA polymerase II elongation factor ELL
Weight
68.265kDa
Superfamily
ELL/occludin family
Alternative Names
C19orf17; chromosome 19 open reading frame 17; DKFZp434I1916; Eleven-nineteen lysine-rich leukemia protein; ELL gene (11-19 lysine-rich leukemia gene); ELL1; elongation factor RNA polymerase II; MEN; RNA polymerase II elongation factor ELL ELL C19orf171, MEN, PPP1R68, ELL elongation factor for RNA polymerase II RNA polymerase II elongation factor ELL|ELL gene (11-19 lysine-rich leukemia gene)|ELL/KMT2A fusion protein|KMT2A/ELL fusion protein|eleven-nineteen lysine-rich leukemia protein|elongation factor RNA polymerase II|protein phosphatase 1, regulatory subunit 68
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ELL, check out the ELL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ELL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ELL Colorimetric Cell-Based ELISA (EKC1753)
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