Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 2 publication(s).
Anti-GAD65/GAD2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-GAD65/GAD2 Antibody
See all GAD2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GAD65/GAD2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GAD65/GAD2 information: Molecular Weight: 65411 MW; Subcellular Localization: Cytoplasm, cytosol . Cytoplasmic vesicle . Cell junction, synapse, presynaptic cell membrane ; Lipid-anchor . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.|
|Cite This Product||Anti-GAD65/GAD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1403)|
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|Anti-GAD65/GAD2 Antibody may replace the following items: sc 130569|sc 32270|sc 365180|sc 377145|sc 53541|sc 5601|sc 73650|sc 7511.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Glutamate decarboxylase 2|
|Subcellular Localization||Cytoplasm, cytosol . Cytoplasmic vesicle . Cell junction, synapse, presynaptic cell membrane ; Lipid-anchor . Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Catalyzes the production of GABA.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-GAD65/GAD2 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
IHC(P): Rat Brain Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...||PubMed ID 21444355|
|Acrylamide neurotoxicity on the cerebrum of weaning rats||PubMed ID 26199611|
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Note: sample size takes longer to process, average 5-7 days.
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