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Product Info Summary
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Anti-Pannexin 2/PANX2 Picoband™ Antibody
Boster Bio Anti-Pannexin 2/PANX2 Picoband™ Antibody catalog # A08860-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pannexin 2/PANX2 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08860-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human Pannexin 2/PANX2 recombinant protein (Position: E19-F677).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A08860-1 is reactive to PANX2 in Human
A08860-1 is guaranteed for ELISA, WB Boster Guarantee
Background of Pannexin-2
Pannexin 2 is a protein that in humans is encoded by the PANX2 gene. It is mapped to 22q13.33. The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 1 are abundantly expressed in central nervous system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 1 may form cell type-specific gap junctions with distinct properties. Multiple transcript variants encoding different isoforms have been found for this gene.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PANX2 using anti-PANX2 antibody (A08860-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: humna SW579 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PANX2 antigen affinity purified polyclonal antibody (Catalog # A08860-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PANX2 at approximately 74KD. The expected band size for PANX2 is at 74KD.
Gene/Protein Information For PANX2 (Source: Uniprot.org, NCBI)
hPANX2; MGC119432; pannexin 2; Pannexin2; Pannexin-2; PANX2; PX2 PANX2 PX2, hPANX2 pannexin 2 pannexin-2*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on PANX2, check out the PANX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PANX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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