Equine IL-10 ELISA Kit PicoKine®

Product Name

Equine IL-10 ELISA Kit PicoKine®

SKU/Catalog Number

EK2361

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Equine IL-10 ELISA Kit PicoKine® (96 Tests). Quantitate Equine IL-10 in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Equine IL-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2361)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: Ser19-Asn178

Capture/Detection Antibodies

polyclonal antibody from goat|polyclonal antibody from goat

Sensitivity

<50 pg/mL

Assay Range

0.3-20 ng/mL

Standard Dilution Instructions

See datasheet of EK2361 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2361 is reactive to IL10 in Equine samples

Validated Sample Types

cell culture supernatants, serum, plasma(heparin, EDTA)

Application Guarantee

EK2361 is guaranteed for ELISA in Equine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL10

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Kit Components

Catalog Number	Description	Quantity
EK2361-CAP	Anti-Equine IL10 Pre-coated 96-well strip microplate	1
EK2361-ST	Equine IL10 Standard	2 vials, 20 ng/tube
EK2361-DA	Equine IL10 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.3	0.63	1.25	2.5	5	10	20
O.D.	0.036	0.088	0.137	0.241	0.406	0.702	1.218	1.888

Data Example Images

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