Equine IL-4 ELISA Kit PicoKine®

Product Name

Equine IL-4 ELISA Kit PicoKine®

SKU/Catalog Number

EK2365

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Equine IL-4 ELISA Kit PicoKine® (96 Tests). Quantitate Equine IL-4 in cell culture supernatants, serum, plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Equine IL-4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2365)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: Lys26-Cys137

Capture/Detection Antibodies

polyclonal antibody from goat|polyclonal antibody from goat

Sensitivity

<10 pg/mL

Assay Range

62.5-4,000 pg/mL

Standard Dilution Instructions

See datasheet of EK2365 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2365 is reactive to IL4R in Equine samples

Validated Sample Types

cell culture supernatants, serum, plasma(heparin, EDTA)

Application Guarantee

EK2365 is guaranteed for ELISA in Equine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL4R

IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Kit Components

Catalog Number	Description	Quantity
EK2365-CAP	Anti-Equine IL4R Pre-coated 96-well strip microplate	1
EK2365-ST	Equine IL4R Standard	2 vials, 10 ng/tube
EK2365-DA	Equine IL4R Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
O.D.	0.037	0.11	0.165	0.267	0.461	0.924	1.741	2.412

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Equine IL-4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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