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Product Info Summary
| SKU: | LS083417 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of Fc receptor-like 4 (FCRL4) |
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Product info
Product Name
FCRL4 (NM_031282) Human Over-expression Lysate
See all FCRL4/FcRH4/IRTA1 products
SKU/Catalog Number
LS083417
Size
100 µg
Description
Transient overexpression lysate of Fc receptor-like 4 (FCRL4)
Storage & Handling
Cite This Product
FCRL4 (NM_031282) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083417)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FCRL4 Human Over-expression Lysates NM_031282.
Western blot validation of overexpression lysate (LS083417) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FCRL4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for FCRL4 (Source: Uniprot.org, NCBI)
Gene Name
FCRL4
Full Name
Fc receptor-like protein 4
Weight
57.224kDa
Alternative Names
CD307d antigen; CD307d; Fc receptor homolog 4; Fc receptor-like 4; FcRH4; FCRH4Fc receptor-like protein 4; FCRL4; FcR-like protein 4; hIFGP2; IFGP family protein 2; IFGP2; IGFP2; Immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; IRTA1; IRTA1MGC150523; MGC150522 FCRL4 CD307d, FCRH4, IGFP2, IRTA1 Fc receptor like 4 Fc receptor-like protein 4|IFGP family protein 2|fc receptor homolog 4|fcR-like protein 4|hIFGP2|immune receptor translocation-associated protein 1|immunoglobulin superfamily Fc receptor, gp42|immunoglobulin superfamily receptor translocation associated 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FCRL4, check out the FCRL4 Infographic
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In this infographic, you will see the following information for FCRL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FCRL4 (NM_031282) Human Over-expression Lysate (LS083417)
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No publications found for LS083417
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