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Product Info Summary
| SKU: | BA1125 |
|---|---|
| Size: | 0.5ml |
| Application: | Flow Cytometry, IF |
Product info
| Product Name | FITC Conjugated Avidin |
| SKU/Catalog Number | BA1125 |
| Pack Size | 0.5 ml |
| Form | Concentrated, Liquid |
| Concentration | 1 mg/ml |
| Product Description | Avidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. |
| Contents | 0.5 mg of FITC conjugated avidin, F/P=3/1, 0.01 M PBS, 5 mg/mL BSA, 50% glycerol. And the isoelectric point of avidin reduced from 10 to 6.5 through chemical modification. Thus, avidin has very low non-specific absorption to tissue and cell. |
| Labeling Method | Avidin is conjugated to FITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457 (1969)) |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Applications | IF, Flow Cytometry |
| Cite This Product | FITC Conjugated Avidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1125) |
Product Images
Validation Images & Assay Conditions
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HADH was detected in a paraffin-embedded section of human intestinal tissues using rabbit anti-HADH Antigen Affinity purified polyclonal antibody (Catalog # A03650-1). Biotin conjugated goat anti-rabbit IgG (BA1003) was used to detect the primary antibody. DyLight®550 Conjugated Avidin (BA1134) was used for visualization.
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