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Product Info Summary
| SKU: | BA1121 |
|---|---|
| Size: | 0.5 mL |
| Application: | Flow Cytometry, IF |
Product info
| Product Name | FITC Conjugated Protein G |
| SKU/Catalog Number | BA1121 |
| Pack Size | 0.5ml |
| Form | Concentrated, Liquid |
| Concentration | 1 mg/mL |
| Description | Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing binding specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fab and Fc region. The native molecule also binds albumin, but because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. |
| Contents | 0.5 mg of FITC conjugated protein G, F/P=3/1; 0.01M PBS; 50% glycerol. |
| Labeling Method | Protein G is conjugated to FITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457 (1969)) |
| Storage & Expiration | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| Applications | IF, Flow Cytometry |
| Cite This Product | FITC Conjugated Protein G (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1121) |
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunofluorescence, 10-20 μg/mL
Flow Cytometry, 10-20 μg/mL
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