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Product Info Summary
|Description:||Transient overexpression lysate of glyoxylate reductase 1 homolog (Arabidopsis) (GLYR1)|
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GLYR1 (NM_032569) Human Over-expression Lysate
Transient overexpression lysate of glyoxylate reductase 1 homolog (Arabidopsis) (GLYR1)
Storage & Handling
Cite This Product
GLYR1 (NM_032569) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084656)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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GLYR1 Human Over-expression Lysates NM_032569.
Western blot validation of overexpression lysate (LS084656) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with GLYR1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for GLYR1 (Source: Uniprot.org, NCBI)
Putative oxidoreductase GLYR1
BM045; Cytokine-like nuclear factor N-PAC; glyoxylate reductase 1 homolog (Arabidopsis); Glyoxylate reductase 1 homolog; HIBDLnuclear protein 60 kDa; NP603-hydroxyisobutyrate dehydrogenase-like protein; N-PAC; nuclear protein 60kDa; Nuclear protein NP60; Nuclear protein of 60 kDa; putative oxidoreductase GLYR1 GLYR1 BM045, HIBDL, N-PAC, NP60, hNDF glyoxylate reductase 1 homolog putative oxidoreductase GLYR1|3-hydroxyisobutyrate dehydrogenase-like protein|cytokine-like nuclear factor n-pac|nuclear protein 60 kDa|nuclear protein 60kDa|nuclear protein NP60|nuclear protein of 60 kDa|nucleosome-destabilizing factor*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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