Product Info Summary
SKU: | LS006928 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of HNF1 homeobox B (HNF1B), transcript variant 1 |
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Product info
Product Name
HNF1 beta (HNF1B) (NM_000458) Human Over-expression Lysate
See all TCF-2/HNF-1 beta products
SKU/Catalog Number
LS006928
Size
100 µg
Description
Transient overexpression lysate of HNF1 homeobox B (HNF1B), transcript variant 1
Storage & Handling
Cite This Product
HNF1 beta (HNF1B) (NM_000458) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006928)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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HNF1 beta (HNF1B) Human Over-expression Lysates NM_000458.
Western blot validation of overexpression lysate (LS006928) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HNF1B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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HNF1 beta (HNF1B) Human Over-expression Lysates NM_001165923.
Western blot validation of overexpression lysate (LS006928) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HNF1B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for HNF1B (Source: Uniprot.org, NCBI)
Gene Name
HNF1B
Full Name
Hepatocyte nuclear factor 1-beta
Weight
61.324kDa
Superfamily
HNF1 homeobox family
Alternative Names
FJHN; hepatocyte nuclear factor 1-beta; HNF1 beta A; HNF-1 beta; HNF1 homeobox B; HNF1B; HNF-1B; HNF1beta; HNF-1-beta; HNF2; Homeoprotein LFB3; LFB3; LF-B3; MODY5; MODY5HPC11; TCF2; TCF-2; TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2; transcription factor 2, hepatic; Variant hepatic nuclear factor 1; VHNF1 HNF1B ADTKD3, FJHN, HNF-1-beta, HNF-1B, HNF1beta, HNF2, HPC11, LF-B3, LFB3, MODY5, RCAD, T2D, TCF-2, TCF2, VHNF1 HNF1 homeobox B hepatocyte nuclear factor 1-beta|HNF1 beta A|homeoprotein LFB3|transcription factor 2, hepatic
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HNF1B, check out the HNF1B Infographic
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In this infographic, you will see the following information for HNF1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For HNF1 beta (HNF1B) (NM_000458) Human Over-expression Lysate (LS006928)
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