HRP Conjugated AffiniPure Rabbit Anti-Avidin

Avidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. This antibody is obtained from the rabbit which was immunized by reconstructed avidin, and it is specific for natural and reconstructed avidin. Cited in 5 publication(s).

Product Info Summary

SKU: BA1082
Size: 0.5ml
Host: Rabbit
Application: ELISA, WB

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Product NameHRP Conjugated AffiniPure Rabbit Anti-Avidin
SKU/Catalog NumberBA1082
Pack Size0.5 ml
FormConcentrated, Liquid
Concentration1mg/ml
Product DescriptionAvidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. This antibody is obtained from the rabbit which was immunized by reconstructed avidin, and it is specific for natural and reconstructed avidin.
Contents0.5 mg of HRP conjugated rabbit anti-avidin
0.01 M PBS (pH7.4)
50% glycerol.
Labeling MethodRabbit anti-avidin is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
(Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
StorageAt -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
ApplicationsWB, ELISA
Cite This ProductPeroxidase Conjugated Rabbit anti-avidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1082)

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.25-0.5μg/ml (ECL detection) Western Blotting: 2.5-5μg/ml (DAB detection) Direct ELISA: 0.01-0.1μg/ml (TMB detection)

Validation Images & Assay Conditions

Hello CJ!

BA1082 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma

5-Hydroxy-4′-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/β-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells

Regulation of Eclipta prostrata L. components on cigarette smoking‐induced autophagy of bronchial epithelial cells via keap1‐Nrf2 pathway

5-Hydroxy-4?-Nitro-7-Propionyloxy-Genistein Inhibited Invasion and Metastasis via Inactivating Wnt/?-Catenin Signal Pathway in Human Endometrial Carcinoma Ji Endometrial Cells

Bai J, Yang BJ, Luo X. Oncol Lett. 2018 Apr;15(4):5227-5235. doi: 10.3892/ol.2018.7938. Epub 2018 Feb 2. Effects of 5-hydroxy-4

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