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Product Info Summary
| SKU: | BA1070 |
|---|---|
| Size: | 0.5ml |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Overview
| ProductName | HRP Conjugated AffiniPure Rabbit Anti-Human IgG (H+L) |
|---|---|
| Synonyms | HRP-conjugated rabbit anti-human IgG |
| Description | HRP Conjugated Rabbit Anti-human IgG (H+L) (gamma-chain specific) secondary antibody This HRP conjugated antibody is specific for human IgG and shows no cross-reactivity with rat/mouse/goat/rabbit IgG./td> |
| Reagent Type | Secondary antibody, reporter enzyme labeled |
| Label | HRP (Horseradish Peroxidase) |
| Host | Rabbit |
| Target Species | Human |
| Antibody Class | IgG |
| Clonality | Polyclonal |
| Immunogen | Human IgG (whole molecular) |
| Preparation | Affinity purified from rabbit antiserum |
| Specificyity | This HRP conjugated antibody is specific for human IgG and shows no cross-reactivity with rat/mouse/goat/rabbit IgG. |
| Form Supplied | Concentrated, Liquid |
| Formulation | 0.5 mg of HRP conjugated specific antibody
0.01 M PBS (pH 7.4) 50% glycerol |
| Pack Size | 0.5 ml |
| Concentration | 1mg/ml |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Application | ELISA*, WB* *Our Boster Guarantee covers the use of this product in the above marked tested applications. |
| Precautions | FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE |
Assay Information
| Sample Type | |
|---|---|
| Assay Type | SDS-PAGE separated-, membrane-immobilized-, human primary-antibody-probed proteins from cell/tissue lysates |
| Assay Purpose | Protein detection |
| Technique | Immunodetection of target antibody with HRP reporter enzyme |
| Equipment Needed | WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer |
| Compatibility with Reagents | Incompatible with sodium azide and metals incompatible with high phosphate concentrations |
Main Advantages
| Specific | High signal-to-noise ratio |
|---|---|
| Sensitive | Detects low-abundant targets due to an optimal number of HRP molecules per antibody |
| High Signal Amplification | Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC |
| Fast | Generates strong signals in a relatively short time span |
| Quantifieable | Allows quantification of detected signal |
| Easy to Use | Supplied in a workable liquid format |
| Flexible | HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates; |
| Convenient | HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes |
Background
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.
Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.
Product Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) Direct ELISA: 0.05-0.5μg/ml (TMB detection)
Validation Images & Assay Conditions
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Specific Publications For BA1070
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BA1070 has been cited in 92 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miRNA‑15a‑5p facilitates the bone marrow stem cell apoptosis of femoral head necrosis through the Wnt/β‑catenin/PPARγ signaling pathway
In the Search of Potential Serodiagnostic Proteins to Discriminate Between Acute and Chronic Q Fever in Humans. Some Promising Outcomes
Molecular characterization of cathepsin B from Clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis
HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways:
Effects of Ureaplasma urealyticum lipid-associated membrane proteins on rheumatoid arthritis synovial fibroblasts:
Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells
Antigenicity and immunogenicity of HIV-1 gp140 with different combinations of glycan mutation and V1/V2 region or V3 crown deletion
Screening and identification of a novel B-cell neutralizing epitope from Helicobacter pylori UreB
TGF-β1 and iNOS signaling were involved in the effect of Prostaglandin E2 on progression of lower limb varicose veins
Molecular characterization and evolutionary analysis of horse BAFF-R, a tumor necrosis factor receptor related to B-cell survival
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1 Customer Q&As for HRP Conjugated AffiniPure Rabbit Anti-Human IgG (H+L)
Question
Could you help provide a detailed response to his questions. I want to know if the antibody BA1070 can detect primary Antibody against Apo-lipoprotein E4 made in the host - Mouse? BA1070 will not detect, will need an anti-mouse secondary antibody. What exactly does Anti-IgG detect? If I run some crude lysate and for its detection I use specific anti-protein antibody as the primary. Can I use Anti-IgG as a secondary?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
I want to know if the antibody BA1070 can detect primary Antibody against Apo-lipoprotein E4 made in the host - Mouse? BA1070 will not detect, will need an anti-mouse secondary antibody. What exactly does Anti-IgG detect? Anti-IgG detect IgG. For example, BA1070 is
Boster Scientific Support
Answered: 2017-10-02
