Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)

Product Name

Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)

SKU/Catalog Number

EK7102

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human 25 (OH) Vitamin D ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human 25(OH) Vitamin D ELISA Kit (Competitive EIA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7102)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.5 ng/ml

Assay Range

2.5-150 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7102 is reactive to in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7102 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
1. Microwell plate coated with anti-Vitamin D	12x8x1
2. Vitamin D Standard Set: 7 vials (ready to use)	0.5 ml
3. Vitamin D Control Set: 2 vials (ready to use)	0.5 ml
4. Biotinylated 25 (OH)D Reagent: 1 Vial (51X)	0.55 ml
5. Assay Diluent, 1 bottle	24 ml
6. Streptavidin-HRP, 1 bottle (ready to use)	23 mL
7. Stop Solution, 1 bottle (ready to use)	12 mL
8. TMB Substrate, 2 bottles (ready to use)	2 x 12 ml
9. Microplate sealing film	2
10. Wash Concentrate 20X, 1 bottle	25 ml

Standard Concentrations and example data

	OD 450 nm	25(OH)D, (ng/ml)
Std 1	2.4	0
Std 2	2.1	2.5
Std 3	1.76	5
Std 4	1.31	15
Std 5	0.79	35
Std 6	0.48	70
Std 7	0.29	150

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, ELISA Target remove.

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