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Product Info Summary
| SKU: | EK2138 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ACP5 ELISA Kit PicoKine®
View all TRACP/PAP/ACP5 ELISA kits
SKU/Catalog Number
EK2138
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ACP5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ACP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ACP5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2138)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A22-P325
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2138 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2138 is reactive to ACP5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2138 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRACP/PAP/ACP5
Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2138-CAP | Anti-Human ACP5 Pre-coated 96-well strip microplate | 1 |
| EK2138-ST | Human ACP5 Standard | 2 vials, 10 ng/tube |
| EK2138-DA | Human ACP5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.4 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.041 | 0.085 | 0.191 | 0.391 | 0.864 | 1.230 | 1.863 | 2.368 |
Data Example Images
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Human ACP5 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRACP/PAP/ACP5, Dilution ratio of 1:1, concentration in serum and plasma is around 600 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ACP5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 84 | 352 | 865 | 78 | 296 | 843 |
| Standard deviation | 4.54 | 20.77 | 36.33 | 3.35 | 17.17 | 52.27 |
| CV(%) | 5.4% | 5.9% | 4.2% | 4.3% | 5.8% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TRACP/PAP/ACP5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 92 | 94 | 98 | 92 | 3.5 | 3.8% |
| Sample 2 | 352 | 342 | 331 | 295 | 330 | 13.86 | 4.2% |
| Sample 3 | 865 | 875 | 795 | 791 | 832 | 45.73 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For ACP5 (Source: Uniprot.Org, NCBI)
Gene Name
ACP5
Full Name
Tartrate-resistant acid phosphatase type 5
Weight
36.599kDa
Superfamily
metallophosphoesterase superfamily
Alternative Names
ACP5; TRACP; TRACP-5b; TTRRAP ACP5 HPAP, TRACP5a, TRACP5b, TRAP, TrATPase acid phosphatase 5, tartrate resistant tartrate-resistant acid phosphatase type 5|human purple acid phosphatase|tartrate-resistant acid ATPase|tartrate-resistant acid phosphatase 5a|tartrate-resistant acid phosphatase 5b
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACP5, check out the ACP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ACP5 ELISA Kit PicoKine® (EK2138)
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