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Product Info Summary
| SKU: | EK0301 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Human Activin A ELISA Kit PicoKine®
SKU/Catalog Number
EK0301
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Human INHBA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Activin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0301)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: G311-S426
Sensitivity
<12 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0301 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0301 is reactive to INHBA in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0301 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Activin A
Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0301-CAP | Anti-Human INHBA Pre-coated 96-well strip microplate | 1 |
| EK0301-ST | Human INHBA Standard | 2 vials, 10 ng/tube |
| EK0301-DA | Human INHBA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.048 | 0.094 | 0.132 | 0.201 | 0.384 | 0.692 | 1.395 | 2.013 |
Data Example Images
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Human Activin A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Activin A, Dilution ratio of 1:1, concentration in serum and plasma is 113-903 pg/ml..
Some articles we found to cite concentrations of Activin A in samples: 24447483, 20457668 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Activin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 199 | 467 | 33 | 205 | 482 |
| Standard deviation | 1.63 | 15.72 | 29.88 | 2.54 | 14.76 | 37.96 |
| CV (%) | 4.8% | 7.9% | 6.4% | 7.7% | 7.2% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Activin A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 40 | 35 | 33 | 35 | 2.69 | 7.6% |
| Sample 2 | 199 | 204 | 220 | 225 | 212 | 10.79 | 5% |
| Sample 3 | 467 | 440 | 464 | 464 | 458 | 10.89 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For INHBA (Source: Uniprot.Org, NCBI)
Gene Name
INHBA
Full Name
Inhibin beta A chain
Weight
47.442kDa
Superfamily
TGF-beta family
Alternative Names
Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1 INHBA EDF, FRP inhibin subunit beta A inhibin beta A chain|FSH-releasing protein|Inhibin, beta-1|activin beta-A chain|erythroid differentiation factor|erythroid differentiation protein|follicle-stimulating hormone-releasing protein|inhibin beta A subunit|inhibin, beta A (activin A, activin AB alpha polypeptide)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on INHBA, check out the INHBA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for INHBA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Activin A ELISA Kit PicoKine® (EK0301)
Hello CJ!
EK0301 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of general anesthesia on serum β-amyloid protein and regional cerebral oxygen saturation of elderly patients after subtotal gastrectomy
Research on changes in cognitive function, β-amyloid peptide and neurotrophic factor in stroke patients.
Changes in Blood Components in Aphtha Patients with Excess Heat
Changes in Blood Components in Aphtha Patients with Excess Heat
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human Activin A ELISA Kit PicoKine®
Question
How should the standard 1 be diluted if there is no liquid provided for EK0301?
Verified customer
Asked: 2020-06-24
Answer
For the Human Activin A ELISA Kit PicoKine™ (EK0301), reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent.
Boster Scientific Support
Answered: 2020-06-28
Question
What can be used as a wash buffer for EK0301?
Verified customer
Asked: 2020-06-24
Answer
For the Human Activin A ELISA Kit PicoKine™ (EK0301), preparation of wash buffer: Add 8.5g Nacl, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6.
Boster Scientific Support
Answered: 2020-06-28
Question
Q: which procedure should I follow in order to thaw whole blood sample for Activin A ELISA after freezing?
S. Bhatt
Verified customer
Asked: 2020-03-31
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Activin A for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-03-31
Question
Q: how to analyze ELISA data? I have performed Activin A level in serum.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-07-02
Question
Q: is it okay to use citrate plasma as samples in Human Activin A Picokine® ELISA Kit (Catalog # EK0301)?
Verified Customer
Verified customer
Asked: 2019-06-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Activin A causing disruption of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-06-23
Question
Q: if the enzyme conjugated Activin A antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Activin A antigen?
Verified Customer
Verified customer
Asked: 2019-02-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-16
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Activin A in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
R. Scott
Verified customer
Asked: 2018-09-26
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Activin A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-09-26
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
P. Jones
Verified customer
Asked: 2018-04-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-09
