Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine®

AgRP/ART ELISA kit for Human

Human AGRP/agouti-related ELISA Kit PicoKine™ (96 Tests). Quantitate Human AGRP in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 4pg/ml.

Product Info Summary

SKU: EK1427
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Product Name

Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine®

View all AgRP/ART ELISA kits

SKU/Catalog Number

EK1427

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human AGRP/agouti-related ELISA Kit PicoKine™ (96 Tests). Quantitate Human AGRP in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1427)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A21-T132

Sensitivity

<4 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1427 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1427 is reactive to AGRP in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK1427 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of AgRP/ART

Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Kit Components

Catalog Number Description Quantity
EK1427-CAP Anti-Human AGRP Pre-coated 96-well strip microplate 1
EK1427-ST Human AGRP Standard 2 vials, 10 ng/tube
EK1427-DA Human AGRP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0120.0650.1130.2230.4400.8141.4392.332

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)159923613102246
Standard deviation0.633.9610.380.685.412.05
CV (%)4.2%4%4.4%5.3%5.3%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of AgRP/ART in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 115181617161.116.9%
Sample 2991009395962.862.9%
Sample 323621820223322213.536%
*number of samples for each test n=16.

Gene/Protein Information For AGRP (Source: Uniprot.Org, NCBI)

Gene Name

AGRP

Full Name

Agouti-related protein

Weight

14.44kDa

Alternative Names

agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2 AGRP AGRT, ART, ASIP2 agouti related neuropeptide agouti-related protein|agouti related protein homolog

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AGRP, check out the AGRP Infographic

AGRP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for AGRP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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7 Customer Q&As for Human AGRP/ART/Agouti-related protein ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-10-13

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-10-13

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-07-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-07

Question

Q: how to analyze ELISA data? I have performed AgRP level in urine.

S. Phillips

Verified customer

Asked: 2020-06-14

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-06-14

Question

Q: can I use EDTA plasma as samples in Human AgRP Picokine® ELISA Kit (Catalog # EK1427)?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of AgRP causing degradation of its protein structure. AgRP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the AgRP ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-03-13

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of AgRP in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the AgRP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-06

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-11-07

Question

Q: if the enzyme conjugated AgRP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the AgRP antigen?

K. Babu

Verified customer

Asked: 2018-08-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-08-10

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