Human alpha Fetoprotein OneStep ELISA Kit (AFP)

Product Name

Human alpha Fetoprotein OneStep ELISA Kit (AFP)

See all AFP products

SKU/Catalog Number

EK7020

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human AFP. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human alpha Fetoprotein OneStep ELISA Kit (AFP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7020)

Clonality of Antibodies

See Datasheet for details

Sensitivity

3 IU/ml

Assay Range

5-200 IU/mL

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7020 is reactive to AFP in Human samples

Validated Sample Types

Serum and Plasma (heparin, citrate)

Application Guarantee

EK7020 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human AFP Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti-AFP monoclonal antibody, Polystyrene micro-well plate
Human AFP Standards (S0~S6)	7	lml	AFP (0, 5, 10, 20, 50, 100, 200) IU/ml, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein
HRP Conjugated anti-Human AFP antibody	1	7ml	HRP Conjugated anti-Human AFP antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300, from mouse monoclonal antibody
Controls	2	1ml	L (10.5-19.5) H (46.3-86.0) IU/ml, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein
20X Wash Buffer Concentrate	1	15ml	Phosphorous salts
Color Developing Reagent A	1	7ml	Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	TMB
Stop Solution	1	7ml	Dilute sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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