Human alpha Fetoprotein OneStep ELISA Kit (AFP)

alpha-Fetoprotein/AFP ELISA kit

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human AFP. 96 wells/kit.

Product Info Summary

SKU: EK7020
Size: 96 wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum and Plasma (heparin, citrate)

Product Name

Human alpha Fetoprotein OneStep ELISA Kit (AFP)

See all alpha-Fetoprotein/AFP products

SKU/Catalog Number

EK7020

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human AFP. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human alpha Fetoprotein OneStep ELISA Kit (AFP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7020)

Clonality of Antibodies

See Datasheet for details

Sensitivity

3 IU/ml

Assay Range

5-200 IU/mL

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7020 is reactive to AFP in Human samples

Validated Sample Types

Serum and Plasma (heparin, citrate)

Application Guarantee

EK7020 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Anti-Human AFP Pre-coated 96-well strip microplate18 strips of 12 wellsAnti-AFP monoclonal antibody, Polystyrene micro-well plate
Human AFP Standards (S0~S6)7lmlAFP (0, 5, 10, 20, 50, 100, 200) IU/ml, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein
HRP Conjugated anti-Human AFP antibody17mlHRP Conjugated anti-Human AFP antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300, from mouse monoclonal antibody
Controls21mlL (10.5-19.5) H (46.3-86.0) IU/ml, 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300, from natural protein
20X Wash Buffer Concentrate115mlPhosphorous salts
Color Developing Reagent A17mlUrea hydrogen peroxide
Color Developing Reagent B17mlTMB
Stop Solution17mlDilute sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Gene/Protein Information For AFP (Source: Uniprot.org, NCBI)

Gene Name

AFP

Full Name

Alpha-fetoprotein

Weight

68.678kDa

Superfamily

ALB/AFP/VDB family

Alternative Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AFP, check out the AFP Infographic

AFP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AFP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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2 Customer Q&As for Human alpha Fetoprotein OneStep ELISA Kit (AFP)

Question

I purchased antibodies for detecting AFP in serum by ELISA assay EK7020. But, I want to know the dilution I should used for these antibodies to optimize my ELISA assay in measuring AFP level in serum?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

The dilution of EK7020 is listed on the datasheets and a few things to keep in mind that you do not need to put a lot of antibody to do an ELISA just enough to coat the wells. You could start with 0.1ug/ml. ELISA can be tricky and it take sometimes to optimize your assay.

Boster Scientific Support

Answered: 2020-04-22

Question

I searched on your website and found Human alpha Fetoprotein OneStep ELISA Kit (AFP) and I am interested to purchase it, if it is FDA approved for research purposes. Please let me know.

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The Human alpha Fetoprotein OneStep ELISA Kit (AFP) does not have FDA approval because it is not necessary, since the kits is only to be used for research purposes only. Our products are not intended for diagnostic of therapeutic used.

Boster Scientific Support

Answered: 2019-05-09

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