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Product Info Summary
| SKU: | FEK2103 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human APLP1 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK2103
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human APLP1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APLP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human APLP1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK2103)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S39-E580
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK2103 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK2103 is reactive to APLP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
FEK2103 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of APLP-1
This gene encodes a member of the highly conserved amyloid precursor protein gene family. It is mapped to 19q13.12. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK2103-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK2103-ST | Human APLP-1 Standard | 2 | 10 ng/tube |
| FEK2103-A | Human APLP1 Antibody A | 1 | 3ml |
| FEK2103-B | Human APLP1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.063 | 0.182 | 0.241 | 0.346 | 0.585 | 0.961 | 1.504 | 2.163 |
Data Example Images
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Human APLP1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 270 | 972 | 3395 | 311 | 1030 | 4066 |
| Standard deviation | 11.34 | 43.47 | 166.35 | 15.86 | 66.95 | 300.88 |
| CV(%) | 4.2% | 4.5% | 4.9% | 5.1% | 6.5% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of APLP-1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 270 | 211 | 253 | 236 | 183 | 13.90 | 7.6% |
| Sample 2 | 972 | 1440 | 1371 | 1396 | 1294 | 54.34 | 4.2% |
| Sample 3 | 3395 | 4159 | 3479 | 3643 | 3669 | 194.45 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For APLP1 (Source: Uniprot.Org, NCBI)
Gene Name
APLP1
Full Name
Amyloid-like protein 1
Weight
72.176kDa
Superfamily
APP family
Alternative Names
amyloid beta (A4) precursor-like protein 1; amyloid precursor-like protein 1; amyloid-like protein 1; APLP1; APLP-1; APLPAPLP-1 APLP1 APLP amyloid beta precursor like protein 1 amyloid-like protein 1|amyloid beta (A4) precursor-like protein 1|amyloid precursor-like protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APLP1, check out the APLP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APLP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human APLP1 PicoKine® Quick ELISA Kit (FEK2103)
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