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Product Info Summary
| SKU: | FEK1456 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit
See all Apolipoprotein A-I/ApoA1 products
SKU/Catalog Number
FEK1456
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human APOA1/Apolipoprotein A-I PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human APOA1/Apolipoprotein A-I in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1456)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: D25-Q267
Sensitivity
<50 pg/ml
Assay Range
3.12 ng/ml - 200 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1456 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1456 is reactive to APOA1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK1456 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Apolipoprotein A-I/ApoA1
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high absorbance lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1456-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1456-ST | Human Apolipoprotein A-I/ApoA1 Standard | 2 | 200 ng/tube |
| FEK1456-A | Human APOA1 Antibody A | 1 | 3ml |
| FEK1456-B | Human APOA1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.021 | 0.157 | 0.271 | 0.483 | 0.814 | 1.311 | 1.870 | 2.285 |
Data Example Images
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Human APOA1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 8188 | 19829 | 93784 | 8185 | 19184 | 85441 |
| Standard deviation | 368.46 | 1249.22 | 5533.25 | 532.02 | 1477.16 | 5382.78 |
| CV(%) | 4.5% | 6.3% | 5.9% | 6.5% | 7.7% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Apolipoprotein A-I/ApoA1 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 8188 | 7998 | 8004 | 7332 | 7880 | 330.96 | 4.2% |
| Sample 2 | 19829 | 19750 | 20658 | 19659 | 19974 | 619.19 | 3.1% |
| Sample 3 | 93784 | 86245 | 87457 | 88574 | 89015 | 4005.67 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For APOA1 (Source: Uniprot.Org, NCBI)
Gene Name
APOA1
Full Name
Apolipoprotein A-I
Weight
30.778kDa
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2 APOA1 HPALP2, apo(a) apolipoprotein A1 apolipoprotein A-I|apo-AI|epididymis secretory sperm binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APOA1, check out the APOA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APOA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human APOA1/Apolipoprotein A-I PicoKine® Quick ELISA Kit (FEK1456)
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