Product Info Summary
SKU: | A00717-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Apolipoprotein A I DyLight® 488 conjugated APOA1 Antibody
View all Apolipoprotein A-I/ApoA1 Antibodies
SKU/Catalog Number
A00717-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Apolipoprotein A I DyLight® 488 conjugated APOA1 Antibody catalog # A00717-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Apolipoprotein A I DyLight® 488 conjugated APOA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00717-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Apolipoprotein A I recombinant protein (Position: D25-Q267). Human Apolipoprotein A I shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat Apolipoprotein A I, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00717-Dyl488 is reactive to APOA1 in Human
Applications
A00717-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
30.778kDa
Background of Apolipoprotein A-I/ApoA1
Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of HEPG2 cells using anti-Human Apolipoprotein A I antibody (A00717-Dyl488).
Overlay histogram showing K562 cells stained with A00730-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bid Antibody (A00730-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For APOA1 (Source: Uniprot.org, NCBI)
Gene Name
APOA1
Full Name
Apolipoprotein A-I
Weight
30.778kDa
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2 APOA1 HPALP2, apo(a) apolipoprotein A1 apolipoprotein A-I|apo-AI|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOA1, check out the APOA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Apolipoprotein A I DyLight® 488 conjugated APOA1 Antibody (A00717-Dyl488)
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