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Product Info Summary
| SKU: | M00717-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5)
SKU/Catalog Number
M00717-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5) catalog # M00717-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00717-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
17G5
Isotype
Mouse IgG1
Immunogen
E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264). Mouse Apolipoprotein A I shares 64% and 68.6% amino acid (aa) sequence identity with human and rat Apolipoprotein A I, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00717-1 is reactive to APOA1 in Human
Observed Molecular Weight
25 kDa
Calculated molecular weight
30.8 kDa
Background of APOA1
Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00717-1 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WBhuman HepG2 whole cell, human CACO-2 whole cell
Validation Images & Assay Conditions
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Western blot analysis of APOA1 using anti-APOA1 antibody (M00717-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APOA1 antigen affinity purified monoclonal antibody (M00717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1051) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 25 kDa. The expected band size for APOA1 is at 31 kDa.
Specific Publications For Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5) (M00717-1)
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