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Product Info Summary
| SKU: | EK2135 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
Product info
Product Name
Human Attractin ELISA Kit PicoKine®
SKU/Catalog Number
EK2135
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Attractin ELISA Kit PicoKine® (96 Tests). Quantitate Human ATRN in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Attractin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2135)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A84-Q1272
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK2135 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2135 is reactive to ATRN in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Application Guarantee
EK2135 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ATRN
Attractin is a protein that in humans is encoded by the ATRN gene. This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2135-CAP | Anti-Human ATRN Pre-coated 96-well strip microplate | 1 |
| EK2135-ST | Human ATRN Standard | 2 vials, 50 ng/tube |
| EK2135-DA | Human ATRN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.033 | 0.075 | 0.154 | 0.281 | 0.498 | 0.937 | 1.559 | 2.039 |
Data Example Images
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Human Attractin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ATRN, Dilution ratio of 1:5000, concentration in serum and plasma is 27.8-71 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Attractin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1352 | 5864 | 26013 | 1363 | 6055 | 26542 |
| Standard deviation | 85.18 | 363.57 | 1586.79 | 94.05 | 387.52 | 1353.64 |
| CV(%) | 6.3% | 6.2% | 6.1% | 6.9 | 6.4% | 5.1% |
Specific Publications For Human Attractin ELISA Kit PicoKine® (EK2135)
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