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Product Info Summary
| SKU: | FEK0751 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0751
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EMMPRIN/CD147/BSG in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0751)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T25-H205
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0751 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0751 is reactive to BSG in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk
Application Guarantee
FEK0751 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BSG
Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147(cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition(Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin(CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0751-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0751-ST | Human BSG Standard | 2 | 10 ng/tube |
| FEK0751-A | Human BSG Antibody A | 1 | 3ml |
| FEK0751-B | Human BSG Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.029 | 0.097 | 0.155 | 0.262 | 0.484 | 0.879 | 1.548 | 2.261 |
Data Example Images
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Human BSG PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 71 | 234 | 1067 | 72 | 212 | 977 |
| Standard deviation | 4.68 | 16.84 | 84.29 | 5.40 | 15.90 | 79.13 |
| CV(%) | 6.6% | 7.2% | 7.9% | 7.5% | 7.7% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BSG in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 71 | 71 | 66 | 67 | 68 | 2.58 | 3.8% |
| Sample 2 | 234 | 209 | 223 | 212 | 219 | 11.38 | 5.2% |
| Sample 3 | 1067 | 994 | 957 | 1024 | 1010 | 45.45 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For BSG (Source: Uniprot.Org, NCBI)
Gene Name
BSG
Full Name
Basigin
Weight
42.2kDa
Alternative Names
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor BSG 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, SLC7A11, TCSF basigin (Ok blood group) basigin|OK blood group |collagenase stimulatory factor|extracellular matrix metalloproteinase inducer|hepatoma-associated |leukocyte activation M6|tumor cell-derived collagenase stimulatory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BSG, check out the BSG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BSG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (FEK0751)
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