Human Complement C1R ELISA Kit PicoKine™

Human Complement C1R ELISA Kit PicoKine™ (96 Tests). Quantitate Human C1R in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1954
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Product Name

Human Complement C1R ELISA Kit PicoKine™

See all Complement Component C1r products

SKU/Catalog Number

EK1954

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Complement C1R ELISA Kit PicoKine™ (96 Tests). Quantitate Human C1R in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Complement C1R ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1954)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Sensitivity

<0.1 ng/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1954 is reactive to C1R in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EK1954 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1954-CAP Anti-Human C1R Pre-coated 96-well strip microplate 1
EK1954-ST Human C1R Standard 2 vials, 100 ng/tube
EK1954-DA Human C1R Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.0510.0960.1270.2070.3630.6461.1151.716

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Complement Component C1r, Dilution ratio of 1:500, concentration in serum is around 10ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Complement C1R ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2705112244247530581198742819
Standard deviation129.84583.651953.85186.54707.232740.42
CV (%)4.8%5.2%4.6%6.1%5.9%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Complement Component C1r in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12705282426772767274356.862.0%
Sample 21122412397119141112111664521.594.4%
Sample 342475489014348850585463623451.407.4%
*number of samples for each test n=16.

Gene/Protein Information For C1R (Source: Uniprot.Org, NCBI)

Uniprot ID

P00736

Gene ID

715

Gene Name

C1R

Full Name

Complement C1r subcomponent

Weight

80.119kDa

Superfamily

peptidase S1 family

Alternative Names

C1r; complement C1r subcomponent; Complement component 1 subcomponent r; complement component 1, r subcomponent; Complement Component C1r; EC 3.4.21; EC 3.4.21.41 C1R EDSPD1 complement C1r complement C1r subcomponent|complement component 1, r subcomponent

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on C1R, check out the C1R Infographic

C1R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for C1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human Complement C1R ELISA Kit PicoKine™

Question

Q: is it okay to use EDTA plasma as samples in Human Complement Component C1r Picokine® ELISA Kit (Catalog # EK1954)?

D. Mitchell

Verified customer

Asked: 2020-08-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Complement Component C1r causing disruption of its protein structure. Complement Component C1r may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Complement Component C1r ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-23

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-08-03

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-08-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

S. Allen

Verified customer

Asked: 2019-02-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-02-11

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Complement Component C1r using the EK1954 Human Complement Component C1r Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-02-26

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2017-02-26

Question

Q: Can Complement Component C1r ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2014-10-09

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-10-09

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