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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.|
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Human Complement C1R ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Complement C1R ELISA Kit PicoKine™ (96 Tests). Quantitate Human C1R in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Complement C1R ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1954)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S18-D705
1.56 ng/ml - 100 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1954 is reactive to C1R in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.
EK1954 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Complement Component C1r
Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. It is mapped to 12p13.31. In humans, C1r is encoded by the C1R gene. C1r along with C1q and C1s form the C1 complex, which is the first component of the serum complement system. C1r is an enzyme that activates C1s to its active form, by proteolytic cleavage.C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.
|EK1954-CAP||Anti-Human C1R Pre-coated 96-well strip microplate||1|
|EK1954-ST||Human C1R Standard||2 vials, 100 ng/tube|
|EK1954-DA||Human C1R Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human C1R PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Complement Component C1r, Dilution ratio of 1:500, concentration in serum is around 10ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Complement C1R ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Complement Component C1r in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For C1R (Source: Uniprot.Org, NCBI)
Complement C1r subcomponent
peptidase S1 family
C1r; complement C1r subcomponent; Complement component 1 subcomponent r; complement component 1, r subcomponent; Complement Component C1r; EC 3.4.21; EC 184.108.40.206 C1R EDSPD1 complement C1r complement C1r subcomponent|complement component 1, r subcomponent*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on C1R, check out the C1R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for C1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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