Product Info Summary
SKU: | EK7029 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human CA-125 OneStep ELISA Kit
SKU/Catalog Number
EK7029
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA125. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CA-125 OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7029)
Clonality of Antibodies
See Datasheet for details
Sensitivity
3.8 U/ml
Assay Range
15-300 U/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7029 is reactive to MUC16 in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7029 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Anti-Human CA125 Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- CA125 monoclonal antibody, Polystyrene micro-well plate |
Human CA125 Standards (S1~S4) | 4 | 0.5ml | CA125 (from natural protein) (15,41,120, 300) U/ml, 0.02M PBS, 20% new-born calf serum |
HRP Conjugated anti-Human CA125 antibody | 1 | 6ml | HRP Conjugated anti-Human CA125 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody |
Controls | 1 | 0.5ml | CA125 (from natural protein), 0.02M PBS, 20% new-born calf serum |
20X Wash Buffer Concentrate | 1 | 15ml | Neutral buffer, contains 0.5% Tween-20 |
Color Developing Reagent A | 1 | 7ml | Contains 11mmol/L H2O2 |
Color Developing Reagent B | 1 | 7ml | Contains 2mmol/L TMB |
Stop Solution | 1 | 7ml | 2mol/L dilute sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Click image to see more details
Human CA125 ELISA Kit standard curve
Protein Target Info & Infographic
Gene/Protein Information For MUC16 (Source: Uniprot.org, NCBI)
Gene Name
MUC16
Full Name
Mucin-16
Weight
1519.175kDa
Alternative Names
CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125 MUC16 CA125 mucin 16, cell surface associated mucin-16|CA125 ovarian cancer antigen|cancer antigen 125|ovarian cancer-related tumor marker CA125|ovarian carcinoma antigen CA125
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC16, check out the MUC16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human CA-125 OneStep ELISA Kit (EK7029)
Hello CJ!
EK7029 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li W, Zhang X, Wang J, Li M, Cao C, Tan J, Ma D, Gao Q. Oncotarget. 2017 Oct 6;8(56):96035-96047. doi: 10.18632/oncotarget.21635. eCollection 2017 Nov 10. TGFβ1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian ...
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