Human CA-125 OneStep ELISA Kit

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA125. 96wells/kit, with removable strips. Cited in 1 publication(s).

Product Info Summary

SKU: EK7029
Size: 96wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum

Product Name

Human CA-125 OneStep ELISA Kit

See all CA125/MUC16 products

SKU/Catalog Number

EK7029

Size

96wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA125. 96wells/kit, with removable strips.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CA-125 OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7029)

Clonality of Antibodies

See Datasheet for details

Sensitivity

3.8 U/ml

Assay Range

15-300 U/ml

Cross Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7029 is reactive to MUC16 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7029 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Anti-Human CA125 Pre-coated 96-well strip microplate18 strips of 12 wellsAnti- CA125 monoclonal antibody, Polystyrene micro-well plate
Human CA125 Standards (S1~S4)40.5mlCA125 (from natural protein) (15,41,120, 300) U/ml, 0.02M PBS, 20% new-born calf serum
HRP Conjugated anti-Human CA125 antibody16mlHRP Conjugated anti-Human CA125 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody
Controls10.5mlCA125 (from natural protein), 0.02M PBS, 20% new-born calf serum
20X Wash Buffer Concentrate115mlNeutral buffer, contains 0.5% Tween-20
Color Developing Reagent A17mlContains 11mmol/L H2O2
Color Developing Reagent B17mlContains 2mmol/L TMB
Stop Solution17ml2mol/L dilute sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Gene/Protein Information For MUC16 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8WXI7

Gene ID

94025

Gene Name

MUC16

Full Name

Mucin-16

Weight

1519.175kDa

Alternative Names

CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125 MUC16 CA125 mucin 16, cell surface associated mucin-16|CA125 ovarian cancer antigen|cancer antigen 125|ovarian cancer-related tumor marker CA125|ovarian carcinoma antigen CA125

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MUC16, check out the MUC16 Infographic

MUC16 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MUC16: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK7029 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li W, Zhang X, Wang J, Li M, Cao C, Tan J, Ma D, Gao Q. Oncotarget. 2017 Oct 6;8(56):96035-96047. doi: 10.18632/oncotarget.21635. eCollection 2017 Nov 10. TGFβ1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian ...

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