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Product Info Summary
| SKU: | EK1471 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. |
Product info
Product Name
Human CA125/MUC16 ELISA Kit PicoKine®
View all CA125/MUC16 ELISA kits
SKU/Catalog Number
EK1471
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CA125/MUC16 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MUC16 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 1.5 U/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CA125/MUC16 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1471)
Clonality of Antibodies
See Datasheet for details
Immunogen
natural protein
Sensitivity
<1.5 U/ml
Assay Range
3.12 U/ml - 200 U/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1471 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1471 is reactive to MUC16 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.
Application Guarantee
EK1471 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CA125/MUC16
CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1471-CAP | Anti-Human MUC16 Pre-coated 96-well strip microplate | 1 |
| EK1471-ST | Human MUC16 Standard | 2 vials, 200 U/tube |
| EK1471-DA | Human MUC16 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (U/ml) | 0 | 3.12 | 6.24 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.114 | 0.284 | 0.406 | 0.601 | 0.9 | 1.321 | 1.747 | 2.073 |
Data Example Images
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Human CA125/MUC16 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CA125/MUC16, Dilution ratio of 1:1, concentration in serum and plasma is 1.448-41.76U/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CA125/MUC16 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (U/ml) | 6 | 8 | 71 | 4 | 10 | 77 |
| Standard deviation | 0.42 | 0.63 | 1.94 | 0.28 | 0.7 | 6.04 |
| CV (%) | 7% | 7.9% | 2.7% | 7% | 7% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CA125/MUC16 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (U/ml) | Lot 2 (U/ml) | Lot 3 (U/ml) | Lot 4 (U/ml) | Mean (U/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 6 | 7 | 7 | 7 | 7 | 0.41 | 6.8% |
| Sample 2 | 8 | 8 | 11 | 11 | 10 | 0.31 | 3.3% |
| Sample 3 | 71 | 64 | 98 | 79 | 78 | 2.56 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For MUC16 (Source: Uniprot.Org, NCBI)
Gene Name
MUC16
Full Name
Mucin-16
Weight
1519.175kDa
Alternative Names
CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125 MUC16 CA125 mucin 16, cell surface associated mucin-16|CA125 ovarian cancer antigen|cancer antigen 125|ovarian cancer-related tumor marker CA125|ovarian carcinoma antigen CA125
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MUC16, check out the MUC16 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MUC16: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CA125/MUC16 ELISA Kit PicoKine® (EK1471)
Hello CJ!
EK1471 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li W, Zhang X, Wang J, Li M, Cao C, Tan J, Ma D, Gao Q. Oncotarget. 2017 Oct 6;8(56):96035-96047. doi: 10.18632/oncotarget.21635. eCollection 2017 Nov 10. TGFβ1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian ...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human CA125/MUC16 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated CA125 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CA125 antigen?
Verified Customer
Verified customer
Asked: 2020-06-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-29
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CA125 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
W. Varadkar
Verified customer
Asked: 2018-11-09
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CA125 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-09
Question
Q: which procedure should I follow in order to thaw whole blood sample for CA125 ELISA after freezing?
W. Adams
Verified customer
Asked: 2018-08-19
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CA125 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-08-19
Question
Good afternoon. I hope that this email finds you well. For the kit EK1471 the source sequence is listed as https://www.uniprot.org/uniprot/Q8WXI7. The immunogen itself is listed as a recombinant protein the encodes aa M21005-Q21992. When I looked at Uniprot, https://www.uniprot.org/uniprot/Q8WXI7, the consensus sequence was only 14,507 amino acids, so could not contain M21005-Q21992. Can you help with the sequence of the immunogen?
Verified Customer
Verified customer
Asked: 2016-10-25
Answer
We made a mistake. The immunogen of the standard in the kits from current lot is natural protein. We have corrected it.
Boster Scientific Support
Answered: 2016-10-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2014-11-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-11-24
Question
Q: how do I analyze ELISA data? I have obtained CA125 level in plasma.
D. Gandhi
Verified customer
Asked: 2014-09-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-09-02
