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Product Info Summary
| SKU: | M01641-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CA125 / MUC16 Monoclonal Antibody
SKU/Catalog Number
M01641-1
BM5743 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CA125 / MUC16 Monoclonal Antibody catalog # M01641-1. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CA125 / MUC16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01641-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CA-125 (MUC16) Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Reactive Species
M01641-1 is reactive to MUC16 in Human
Observed Molecular Weight
1519 kDa
Calculated molecular weight
1,519.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01641-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
IHC: human endometrial cancer tissue
Validation Images & Assay Conditions
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IHC analysis of CA125/MUC16 using anti-CA125/MUC16 antibody (M01641-1).
ITGAE was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CA125/MUC16 Antibody (M01641-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CA125 / MUC16 Monoclonal Antibody (M01641-1)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CA125 / MUC16 Monoclonal Antibody
Question
Do you know if M01641-1 (abways CY8822) can be coupled with aldehyde? Does abways have any suggestions?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
Abways didn't offer any suggestion. They said they didn't run similar experiment with CY8822. Please suggest the customer run a pilot test if they want to do it.
Boster Scientific Support
Answered: 2020-03-20
Question
We are currently using anti-CA125 / MUC16 Monoclonal antibody M01641-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
The anti-CA125 / MUC16 Monoclonal antibody (M01641-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-01
Question
Is this M01641-1 anti-CA125 / MUC16 Monoclonal antibody reactive to the isotypes of MUC16?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
The immunogen of M01641-1 anti-CA125 / MUC16 Monoclonal antibody is A synthesized peptide derived from human CA-125 (MUC16) Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-11
