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Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA15-3. 96wells/kit, with removable strips.
The Boster Human CA15-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CA15-3 with a 96-well strip plate that is pre-coated with antibody specific for CA15-3. The detection antibody is a HRP conjugated antibody specific for CA15-3. The capture antibody is monoclonal antibody from mouse, and the detection antibody is monoclonal antibody from mouse. The kit is analytically validated with ready to use reagents. To measure Human CA15-3, add standards and samples to the wells, then add the HRP conjugated detection antibody. Wash away the unbounded protein and HRP conjugated detection antibody. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. Upon addition of the substrate, the density of the yellow product is linearly propotional to Human CA15-3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CA15-3 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster's ELISA Resource Center at https://www.bosterbio.com/elisatechnical-resource-center.
|Product Name||Human CA15-3 ELISA Kit
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|Storage & Handling||Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA15-3. 96wells/kit, with removable strips.|
|Cite This Product||Human CA15-3 ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7030)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Cross Reactivity||There is no detectable cross-reactivity.|
|NCBI Gene Id||4582|
|Species Of This Entry||Human|
|Alternative Names||MUC1|Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
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*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|Anti-Human CA15-3 Pre-coated 96-well strip microplate||1||8 strips of 12 wells||Anti- CA15-3 monoclonal antibody, Polystyrene micro-well plate|
|Human CA15-3 Standards (S1~S4)||4||0.5ml||CA15-3 (from natural protein) (10，50，150, 300) U/ml, 0.02M PBS, 20% new-born calf serum|
|HRP Conjugated anti-Human CA15-3 antibody||1||11ml||HRP Conjugated anti-Human CA15-3 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody|
|Sample diluent||1||11ml||0.02M PBS, 20% new-born calf serum|
|Controls||1||0.5ml||CA15-3 (from natural protein) U/ml, 0.02M PBS, 20% new-born calf serum|
|20X Wash Buffer Concentrate||1||15ml||Neutral buffer, contains 0.5% Tween-20|
|Color Developing Reagent A||1||7ml||Contains 11mmol/L H2O2|
|Color Developing Reagent B||1||7ml||Contains 2mmol/L TMB|
|Stop Solution||1||7ml||2mol/L dilute sulphuric acid|
This data is generated from a recent batch of EK7030 Human CA15-3 ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product.
We measured random samples of Human CA15-3 ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Human CA15-3 ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
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