Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

N-Cadherin ELISA kit for Human

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0669
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

View all N-Cadherin ELISA kits

SKU/Catalog Number

EK0669

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0669)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D160-A724

Sensitivity

<20 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0669 is reactive to CDH2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0669 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of N-Cadherin

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Kit Components

Catalog Number Description Quantity
EK0669-CAP Anti-Human CDH2 Pre-coated 96-well strip microplate 1
EK0669-ST Human CDH2 Standard 2 vials, 50 ng/tube
EK0669-DA Human CDH2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0190.1210.2140.3840.7321.0201.8102.121

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect N-Cadherin, Dilution ratio of 1:1, concentration in serum is around 1500pg/ml.

Some articles we found to cite concentrations of N-Cadherin in samples: 23799912 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2231172492830811904953
Standard deviation13.1650.40202.0520.3369.02376.43
CV (%)5.9%4.3%4.1%6.6%5.8%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of N-Cadherin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122319321122321212.275.7%
Sample 21172126211461232120346.183.8%
Sample 349285617481654885212345.566.6%
*number of samples for each test n=16.

Gene/Protein Information For CDH2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P19022

Gene ID

1000

Gene Name

CDH2

Full Name

Cadherin-2

Weight

99.809kDa

Alternative Names

cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDH2, check out the CDH2 Infographic

CDH2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDH2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0669 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

sE-cadherin is upregulated in serum of patients with renal cell carcinoma and promotes tumor cell dissemination in vitro

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8 Customer Q&As for Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification N-Cadherin using the EK0669 Human N-Cadherin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-05-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-05-30

Question

Q: Can N-Cadherin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

B. White

Verified customer

Asked: 2020-02-07

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-02-07

Question

Can EK0669 detect soluble N-cadherin?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

EK0669 is able to detect soluble N-cadherin.

Boster Scientific Support

Answered: 2019-06-26

Question

Q: if the enzyme conjugated N-Cadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the N-Cadherin antigen?

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-29

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

T. Chakrabarti

Verified customer

Asked: 2018-06-14

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

R. Thompson

Verified customer

Asked: 2018-06-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-05

Question

Q: can I use citrate plasma as samples in Human N-Cadherin Picokine® ELISA Kit (Catalog # EK0669)?

Verified Customer

Verified customer

Asked: 2018-05-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of N-Cadherin causing degradation of its protein structure. N-Cadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the N-Cadherin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-26

Question

Q: how to proceed with the analysis of ELISA data? I measured N-Cadherin level in cell lysates.

D. Jones

Verified customer

Asked: 2018-04-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-04-25

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