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Product Info Summary
| SKU: | EK0669 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®
SKU/Catalog Number
EK0669
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0669)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D160-A724
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from sheep and the detection antibody is polyclonal antibody from sheep.
Sensitivity
<20 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0669 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0669 is reactive to CDH2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0669 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CDH2
Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0669-CAP | Anti-Human CDH2 Pre-coated 96-well strip microplate | 1 |
| EK0669-ST | Human CDH2 Standard | 2 vials, 10 ng/tube |
| EK0669-DA | Human CDH2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.064 | 0.169 | 0.232 | 0.339 | 0.567 | 0.965 | 1.465 | 1.947 |
Data Example Images
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Human Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CDH2, Dilution ratio of 1:1, concentration in serum and plasma is around 1500 pg/ml..
Some articles we found to cite concentrations of CDH2 in samples: 23799912 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 223 | 1172 | 4928 | 308 | 1190 | 4953 |
| Standard deviation | 13.16 | 50.40 | 202.05 | 20.33 | 69.02 | 376.43 |
| CV (%) | 5.9% | 4.3% | 4.1% | 6.6% | 5.8% | 7.6% |
Specific Publications For Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (EK0669)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification N-Cadherin using the EK0669 Human N-Cadherin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-05-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: Can N-Cadherin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
B. White
Verified customer
Asked: 2020-02-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-02-07
Question
Can EK0669 detect soluble N-cadherin?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
EK0669 is able to detect soluble N-cadherin.
Boster Scientific Support
Answered: 2019-06-26
Question
Q: if the enzyme conjugated N-Cadherin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the N-Cadherin antigen?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-01-29
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
T. Chakrabarti
Verified customer
Asked: 2018-06-14
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
R. Thompson
Verified customer
Asked: 2018-06-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-06-05
Question
Q: can I use citrate plasma as samples in Human N-Cadherin Picokine® ELISA Kit (Catalog # EK0669)?
Verified Customer
Verified customer
Asked: 2018-05-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of N-Cadherin causing degradation of its protein structure. N-Cadherin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the N-Cadherin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-26
Question
Q: how to proceed with the analysis of ELISA data? I measured N-Cadherin level in cell lysates.
D. Jones
Verified customer
Asked: 2018-04-25
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-04-25
