Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Product Name

Human/Canine/Porcine Insulin ELISA Kit PicoKine®

SKU/Catalog Number

EK2287

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2287)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: F25-N110

Capture/Detection Antibodies

The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.

Sensitivity

<2 pmol/L

Assay Range

15.6 pmol/L - 1,000 pmol/L

Standard Dilution Instructions

See datasheet of EK2287 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2287 is reactive to INS in Canine, Human, Porcine samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2287 is guaranteed for ELISA in Canine, Human, Porcine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of INS

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Kit Components

Catalog Number	Description	Quantity
EK2287-CAP	Anti-Canine, Human, Porcine INS Pre-coated 96-well strip microplate	1
EK2287-ST	Canine, Human, Porcine INS Standard	2 vials, 1 pmol/tube
EK2287-DA	Canine, Human, Porcine INS Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pmol/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.004	0.086	0.184	0.294	0.56	0.989	1.621	2.158

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect INS, Dilution ratio of 1:1, concentration in human serum is less than the lowest standard, 15.6 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human/Canine/Porcine Insulin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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