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Product Info Summary
| SKU: | EK2176 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Cathepsin A ELISA Kit PicoKine®
SKU/Catalog Number
EK2176
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin A ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cathepsin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2176)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A29-Y480
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2176 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2176 is reactive to CTSA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2176 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CTSA
Complement factor H-related protein 4 is a protein that in humans is encoded by the CFHR4 gene. This gene is a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the hepatocytes, and composed of highly-related short consensus repeats (SCRs). This protein enhances the cofactor activity of CFH, and is involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism. Alternatively spliced transcript variants encoding different isoforms (varying in the number of SCRs) have been described for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2176-CAP | Anti-Human CTSA Pre-coated 96-well strip microplate | 1 |
| EK2176-ST | Human CTSA Standard | 2 vials, 20 ng/tube |
| EK2176-DA | Human CTSA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.041 | 0.073 | 0.092 | 0.145 | 0.301 | 0.547 | 1.146 | 1.854 |
Data Example Images
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Human Cathepsin A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 304 | 786 | 2752 | 308 | 770 | 2825 |
| Standard deviation | 16.11 | 30.65 | 112.83 | 13.55 | 36.19 | 146.9 |
| CV(%) | 5.3% | 3.9% | 4.1% | 4.4% | 4.7% | 5.2% |
Specific Publications For Human Cathepsin A ELISA Kit PicoKine® (EK2176)
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