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Product Info Summary
| SKU: | EK0670 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Cathepsin B ELISA Kit PicoKine®
View all Cathepsin B ELISA kits
SKU/Catalog Number
EK0670
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cathepsin B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0670)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: R18-I339
Sensitivity
<5 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0670 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0670 is reactive to CTSB in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0670 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Cathepsin B
Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0670-CAP | Anti-Human CTSB Pre-coated 96-well strip microplate | 1 |
| EK0670-ST | Human CTSB Standard | 2 vials, 10 ng/tube |
| EK0670-DA | Human CTSB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.048 | 0.217 | 0.299 | 0.522 | 0.897 | 1.074 | 1.548 | 1.923 |
Data Example Images
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Human Cathepsin B PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Cathepsin B, Dilution ratio of 1:40, concentration in serum and plasma is around 100 ng/ml..
Some articles we found to cite concentrations of Cathepsin B in samples: 22848251 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 252 | 1856 | 4296 | 234 | 1923 | 4064 |
| Standard deviation | 17.64 | 129.92 | 326.49 | 17.85 | 148.07 | 377.95 |
| CV (%) | 7% | 7% | 7.6% | 7.8% | 7.7% | 9.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 252 | 275 | 235 | 272 | 258 | 16.19 | 6.2% |
| Sample 2 | 1856 | 1776 | 1691 | 1826 | 1787 | 62.48 | 3.4% |
| Sample 3 | 4296 | 4210 | 3832 | 4617 | 4238 | 279.56 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For CTSB (Source: Uniprot.Org, NCBI)
Gene Name
CTSB
Full Name
Cathepsin B
Weight
37.822kDa
Superfamily
peptidase C1 family
Alternative Names
APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1 CTSB APPS, CPSB, RECEUP cathepsin B cathepsin B|APP secretase|amyloid precursor protein secretase|cathepsin B1|cysteine protease|epididymis secretory sperm binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CTSB, check out the CTSB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Cathepsin B ELISA Kit PicoKine® (EK0670)
Hello CJ!
EK0670 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and Clinical Implications of Cysteine Cathepsins in Gallbladder Carcinoma
Cathepsin L and B as Potential Markers for Liver Fibrosis: Insights From Patients and Experimental Models
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human Cathepsin B ELISA Kit PicoKine®
Question
Will the phenol red in the media interfere the assay when using EK0670 with cell culture supernatant?
Verified customer
Asked: 2021-01-04
Answer
Phenol red in the media will not interfere with the assay.
Boster Scientific Support
Answered: 2021-01-04
Question
Q: Can Cathepsin B ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-10-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-04
Question
Do you have any data available from actual human plasma or serum testing on this kit? Keyword: results, data
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We detected healthy human plasma/serum samples and the concentration is about 32-137 ng/ml, and the recovery range is about 97%-112%.
Boster Scientific Support
Answered: 2020-04-09
Question
Dear Support: Just an inquiry about the Human Cathepsin B ELISA Kit PicoKine does this antibody detects mature form of Cathepsin B or the uncleaved form or both? Also is the kit good for cell lines samples?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The product Human Cathepsin B ELISA Kit PicoKine can detect both mature form and uncleaved form of Cathepasin B. This antibody have been found to work well in serum, cell culture supernatant, plasma and tissue extracts.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: if the enzyme conjugated Cathepsin B antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cathepsin B antigen?
Verified Customer
Verified customer
Asked: 2019-05-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-02
Question
Q: is it okay to use citrate plasma as samples in Human Cathepsin B Picokine® ELISA Kit (Catalog # EK0670)?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Cathepsin B causing disruption of its protein structure. Cathepsin B may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Cathepsin B ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-19
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-08-02
Question
Q: can you suggest the dilution ratio of serum samples for detection of Cathepsin B in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-03-25
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cathepsin B ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-03-25
