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Product Info Summary
| SKU: | EK0688 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CCL27/CTACK ELISA Kit PicoKine®
View all CCL27/CTACK ELISA kits
SKU/Catalog Number
EK0688
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL27/CTACK ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL27/CTACK ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0688)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: F25-G112
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0688 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0688 is reactive to CCL27 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0688 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL27/CTACK
Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0688-CAP | Anti-Human CCL27 Pre-coated 96-well strip microplate | 1 |
| EK0688-ST | Human CCL27 Standard | 2 vials, 10 ng/tube |
| EK0688-DA | Human CCL27 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.026 | 0.044 | 0.073 | 0.151 | 0.391 | 0.742 | 1.57 | 2.418 |
Data Example Images
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Human CCL27/CTACK PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL27/CTACK, Dilution ratio of 1:1, concentration in serum and plasma is 212-945 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL27/CTACK ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 86 | 240 | 846 | 85 | 239 | 777 |
| Standard deviation | 4.98 | 10.56 | 60.06 | 5.78 | 13.86 | 62.93 |
| CV (%) | 5.8% | 4.4% | 7.1% | 6.8% | 5.8% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL27/CTACK in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 86 | 98 | 95 | 92 | 92 | 4.43 | 4.8% |
| Sample 2 | 240 | 249 | 256 | 245 | 247 | 5.85 | 2.3% |
| Sample 3 | 846 | 852 | 955 | 890 | 885 | 43.39 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For CCL27 (Source: Uniprot.Org, NCBI)
Gene Name
CCL27
Full Name
C-C motif chemokine 27
Weight
12.618kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27 CCL27 ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27 C-C motif chemokine ligand 27 C-C motif chemokine 27|CC chemokine ILC|IL-11 R-alpha-locus chemokine|IL-11 Ralpha-locus chemokine|chemokine (C-C motif) ligand 27|cutaneous T-cell attracting chemokine|skinkine|small inducible cytokine subfamily A (Cys-Cys), member 27|small-inducible cytokine A27
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL27, check out the CCL27 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL27/CTACK ELISA Kit PicoKine® (EK0688)
Hello CJ!
EK0688 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum cytokine and chemokine profiles in patients with alopecia areata
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Human CCL27/CTACK ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Liu
Verified customer
Asked: 2020-02-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-10
Question
Q: how do I analyze ELISA data? I measured CCL27 level in plasma.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-05
Question
Q: Can CCL27 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-13
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CCL27 using the EK0688 Human CCL27 Picokine® ELISA Kit?
P. Young
Verified customer
Asked: 2018-05-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-15
Question
Q: What is the optimal O.D. value for CCL27 ELISA kit? I performed your CCL27 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CCL27 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CCL27 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CCL27 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-03-15
Question
Q: can I use citrate plasma as samples in Human CCL27 Picokine® ELISA Kit (Catalog # EK0688)?
Verified Customer
Verified customer
Asked: 2018-03-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CCL27 causing degradation of its protein structure. CCL27 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CCL27 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-11
Question
Q: if the enzyme conjugated CCL27 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL27 antigen?
C. Campbell
Verified customer
Asked: 2016-07-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-07-26
