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Product Info Summary
| SKU: | EK0450 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human CCL4/MIP-1 Beta ELISA Kit PicoKine®
View all CCL4/MIP-1 beta ELISA kits
SKU/Catalog Number
EK0450
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL4/MIP-1 Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL4/MIP-1 Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0450)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A24-N92
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0450 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0450 is reactive to CCL4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0450 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL4/MIP-1 beta
Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0450-CAP | Anti-Human CCL4 Pre-coated 96-well strip microplate | 1 |
| EK0450-ST | Human CCL4 Standard | 2 vials, 10 ng/tube |
| EK0450-DA | Human CCL4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.008 | 0.041 | 0.093 | 0.155 | 0.346 | 0.651 | 0.909 | 1.939 |
Data Example Images
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Human CCL4/MIP-1 beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL4/MIP-1 beta, Dilution ratio of 1:1, concentration in serum and plasma is 0-300 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL4/MIP-1 Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 13 | 90 | 254 | 12 | 95 | 256 |
| Standard deviation | 0.63 | 4.95 | 13.2 | 0.62 | 6.84 | 15.87 |
| CV (%) | 4.9% | 5.5% | 5.2% | 5.2% | 7.2% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL4/MIP-1 beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 13 | 11 | 13 | 14 | 12 | 1.08 | 9% |
| Sample 2 | 90 | 88 | 90 | 93 | 90 | 1.78 | 1.9% |
| Sample 3 | 254 | 290 | 285 | 250 | 269 | 17.89 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For CCL4 (Source: Uniprot.Org, NCBI)
Gene Name
CCL4
Full Name
C-C motif chemokine 4
Weight
10.212kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2 CCL4 ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4 C-C motif chemokine ligand 4 C-C motif chemokine 4|G-26 T-lymphocyte-secreted protein|MIP-1-beta(1-69)|PAT 744|SIS-gamma|T-cell activation protein 2|chemokine (C-C motif) ligand 4|lymphocyte activation gene 1 protein|macrophage inflammatory protein 1-beta|secreted protein G-26|small inducible cytokine A4 (homologous to mouse Mip-1b)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL4, check out the CCL4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CCL4/MIP-1 Beta ELISA Kit PicoKine® (EK0450)
Hello CJ!
EK0450 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bone intramedullary reaming grafts the fracture site with CD146+ skeletal progenitors and downmodulates the inflammatory environment
CCL3/CCR1 mediates CD14+CD16− circulating monocyte recruitment in knee osteoarthritis progression
Multiple myeloma–related deregulation of bone marrow–derived CD34+ hematopoietic stem and progenitor cells
Species: Human
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human CCL4/MIP-1 Beta ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CCL4 using the EK0450 Human CCL4 Picokine® ELISA Kit?
S. Baker
Verified customer
Asked: 2020-06-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-06-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Lewis
Verified customer
Asked: 2019-10-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-10-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-02
Question
Q: can I use citrate plasma as samples in Human CCL4 Picokine® ELISA Kit (Catalog # EK0450)?
W. Mitchell
Verified customer
Asked: 2018-04-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CCL4 causing disruption of its protein structure. CCL4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CCL4 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-04-28
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-11
Question
Q: if the enzyme conjugated CCL4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL4 antigen?
W. Nelson
Verified customer
Asked: 2017-10-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-30
Question
Q: how do I analyze ELISA data? I have performed CCL4 level in plasma.
Verified Customer
Verified customer
Asked: 2017-03-22
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-03-22
Question
Q: which procedure should I follow in order to thaw whole blood sample for CCL4 ELISA after freezing?
A. Babu
Verified customer
Asked: 2016-09-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-09-04
