Human CD157/BST1 ELISA Kit PicoKine™

Human CD157/BST1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BST1 in cell culture supernatants, serum and plasma(heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2144
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma(heparin, EDTA)

Product Name

Human CD157/BST1 ELISA Kit PicoKine™

See all Bone marrow stromal cell antigen 1/CD157 products

SKU/Catalog Number

EK2144

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD157/BST1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human BST1 in cell culture supernatants, serum and plasma(heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CD157/BST1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2144)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G29-A293

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2144 is reactive to BST1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma(heparin, EDTA)

Application Guarantee

EK2144 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2144-CAP Anti-Human BST1 Pre-coated 96-well strip microplate 1
EK2144-ST Human BST1 Standard 2 vials, 10 ng/tube
EK2144-DA Human BST1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0280.0690.0860.1570.2690.5571.0862.211

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD157/BST1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)125526565140142028625655
Standard deviation72.79108.9185.0495.14148.82277.1
CV(%)5.8%4.1%3.6%6.7%5.2%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bone marrow stromal cell antigen 1/CD157 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11255135412641280128837.362.9%
Sample 226562740289528742791142.355.1%
Sample 351405265533354415295232.974.4%
*number of samples for each test n=16.

Gene/Protein Information For BST1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q10588

Gene ID

683

Gene Name

BST1

Full Name

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2

Weight

35.724kDa

Superfamily

ADP-ribosyl cyclase family

Alternative Names

ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; BP-3; BST1; BST-1; cADPr hydrolase 2; CD157 antigen; CD157; Cyclic ADP-ribose hydrolase 2; EC 3.2.2.5; NAD(+) nucleosidase BST1 CD157 bone marrow stromal cell antigen 1 ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2|ADP-ribosyl cyclase 2|NAD(+) nucleosidase|bone marrow stromal antigen 1|bone marrow stromal cell antigen 1 variant 2|cADPr hydrolase 2|cyclic ADP-ribose hydrolase 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BST1, check out the BST1 Infographic

BST1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BST1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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