Human CD23/FCER2 PicoKine™ ELISA Kit
|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Product Name||Human CD23/FCER2 PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD23/FCER2. 96wells/kit, with removable strips.|
|Cite This Product||Human CD23/FCER2 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0912)|
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility.
|Immunogen||Expression system for standard: NSO; Immunogen sequence: D48-S321|
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|EK0912-CAP||96-well plate precoated with anti-Human FCER2 antibody||1|
|EK0912-ST||lyophilized recombinant Human FCER2 standard||10ng/tube|
|EK0912-DA||biotinylated anti-Human FCER2 antibody||130ul(dilution 1:100)|
|AR1103||Avidin-Biotin-Peroxidase Complex(ABC)||130ul(dilution 1:100)|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human CD23/FCER2 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 20-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Fc fragment of IgE, low affinity II, receptor for (CD23)|
|Alternative Names||Low affinity immunoglobulin epsilon Fc receptor ;FCER2 ;|
|Molecular Weight||36299 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Background||CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.|
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The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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