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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CD300F ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CD300F ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD300LF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD300F ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1982)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: T20-S156
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1982 is reactive to CD300LF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1982 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD300f/LMIR3/CD300LF
CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.
|EK1982-CAP||Anti-Human CD300LF Pre-coated 96-well strip microplate||1|
|EK1982-ST||Human CD300LF Standard||2 vials, 10 ng/tube|
|EK1982-DA||Human CD300LF Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD300F PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD300f/LMIR3/CD300LF, Dilution ratio of 1:1, concentration in serum is around 120pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD300F ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD300f/LMIR3/CD300LF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CD300LF (Source: Uniprot.Org, NCBI)
CMRF35-like molecule 1
CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3 CD300LF CD300f, CLM-1, CLM1, IREM-1, IREM1, IgSF13, LMIR3, NKIR CD300 molecule like family member f CMRF35-like molecule 1|CD300 antigen-like family member F|NK inhibitory receptor|immune receptor expressed on myeloid cells 1|immunoglobin superfamily member 13|immunoglobulin superfamily member 13|inhibitory receptor IREM1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD300LF, check out the CD300LF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD300LF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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