Human CD300LG ELISA Kit PicoKine®

CD300LG/Nepmucin ELISA kit for Human

Human CD300LG ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD300LG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Product Info Summary

SKU: EK2155
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human CD300LG ELISA Kit PicoKine®

View all CD300LG/Nepmucin ELISA kits

SKU/Catalog Number

EK2155

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CD300LG ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD300LG in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CD300LG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2155)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-L249

Sensitivity

<20 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2155 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2155 is reactive to Cd300lg in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2155 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CD300LG/Nepmucin

Members of the CD300-like (CD300L) family, such as CD300LG, are widely expressed on hematopoietic cells. All CD300L proteins are type I cell surface glycoproteins that contain a single immunoglobulin (Ig) V-like domain.

Kit Components

Catalog Number Description Quantity
EK2155-CAP Anti-Human Cd300lg Pre-coated 96-well strip microplate 1
EK2155-ST Human Cd300lg Standard 2 vials, 10 ng/tube
EK2155-DA Human Cd300lg Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.512525050010002000
O.D.0.0180.0460.0840.1630.3250.6691.2581.844

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CD300LG/Nepmucin, Dilution ratio of 1:5, concentration in serum and plasma is around 2 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CD300LG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5620275460159747
Standard deviation2.6911.9238.454.0210.3444.82
CV(%)4.8%5.9%5.1%6.7%6.5%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD300LG/Nepmucin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 156584944521.713.3%
Sample 22021861882081966.863.5%
Sample 375475278983978432.124.1%
*number of samples for each test n=16.

Gene/Protein Information For Cd300lg (Source: Uniprot.Org, NCBI)

Gene Name

Cd300lg

Full Name

CMRF35-like molecule 9

Weight

36.756kDa

Superfamily

CD300 family

Alternative Names

CD300 antigen like family member G; CD300 antigen-like family member G; CD300 molecule like family member g; CD300 molecule-like family member g; CD300g antigen; CD300g; CD300LG; CLM9; CLM-9; CLM9Trem4; CMRF35-like molecule 9; Nepmucin; TREM4; TREM-4; Triggering receptor expressed on myeloid cells 4 Cd300lg|2310016B05Rik, Clm, Clm9, D11Ertd736, D11Ertd736e, nepm|CD300 molecule like family member G|CMRF35-like molecule 9|CD300 antigen like family member G|CLM-9|CMRF-35-like molecule-9|nepmucin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd300lg, check out the Cd300lg Infographic

Cd300lg infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd300lg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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