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Product Info Summary
| SKU: | EK2223 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD51 ELISA Kit PicoKine®
SKU/Catalog Number
EK2223
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD51 PicoKine ELISA Kit® (96 Tests). Quantitate Human CD51 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 47 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD51 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2223)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: F31-V992
Sensitivity
<47 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2223 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2223 is reactive to ITGAV in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2223 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ITGAV
Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2223-CAP | Anti-Human ITGAV Pre-coated 96-well strip microplate | 1 |
| EK2223-ST | Human ITGAV Standard | 2 vials, 10 ng/tube |
| EK2223-DA | Human ITGAV Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1,500 | 3,000 | 6,000 |
| O.D. | 0.031 | 0.069 | 0.143 | 0.315 | 0.692 | 0.926 | 1.425 | 2.054 |
Data Example Images
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Human CD51 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD51 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 179 | 236 | 2233 | 108 | 249 | 2866 |
| Standard deviation | 6.01 | 14.18 | 86.64 | 4.36 | 19 | 95.15 |
| CV(%) | 3.4% | 6% | 3.9% | 4% | 7.6% | 3.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ITGAV in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 179 | 151 | 95 | 120 | 136 | 3.15 | 2.3% |
| Sample 2 | 236 | 204 | 241 | 308 | 247 | 12.81 | 5.2% |
| Sample 3 | 2233 | 1878 | 2332 | 2092 | 2134 | 93.24 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For ITGAV (Source: Uniprot.Org, NCBI)
Gene Name
ITGAV
Full Name
Integrin alpha-V
Weight
116.038kDa
Superfamily
integrin alpha chain family
Alternative Names
CD51; Integrin alpha V beta 5; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR ITGAV CD51, MSK8, VNRA, VTNR integrin subunit alpha V integrin alpha-V|antigen identified by monoclonal antibody L230|integrin alphaVbeta3|integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)|vitronectin receptor subunit alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ITGAV, check out the ITGAV Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITGAV: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD51 ELISA Kit PicoKine® (EK2223)
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