Human Chondroadherin ELISA Kit PicoKine®

Chondroadherin ELISA kit for Human

Human Chondroadherin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHAD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Product Info Summary

SKU: EK2167
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human Chondroadherin ELISA Kit PicoKine®

View all Chondroadherin ELISA kits

SKU/Catalog Number

EK2167

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Chondroadherin ELISA Kit PicoKine™ (96 Tests). Quantitate Human CHAD in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Chondroadherin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2167)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: C23-H359

Sensitivity

<20 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2167 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2167 is reactive to CHAD in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2167 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Chondroadherin

Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages.

Kit Components

Catalog Number Description Quantity
EK2167-CAP Anti-Human CHAD Pre-coated 96-well strip microplate 1
EK2167-ST Human CHAD Standard 2 vials, 20 ng/tube
EK2167-DA Human CHAD Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)03126251,2502,5005,00010,00020,000
O.D.0.0510.1930.4230.8391.2101.7482.3552.541

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Chondroadherin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5412865764352229727652
Standard deviation27.59100.28298.0821.4127.8359.64
CV(%)5.1%3.5%3.9%4.1%4.3%4.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Chondroadherin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 154156260161257925.484.4%
Sample 22865295428112826286483.062.9%
Sample 376437263810675767647290.593.8%
*number of samples for each test n=16.

Gene/Protein Information For CHAD (Source: Uniprot.Org, NCBI)

Gene Name

CHAD

Full Name

Chondroadherin

Weight

40.476kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

CHAD; chondroadherin proteoglycan; Chondroadherin; SLRR4A; SLRR4ACartilage leucine-rich protein CHAD SLRR4A chondroadherin chondroadherin|cartilage leucine-rich protein|chondroadherin proteoglycan

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHAD, check out the CHAD Infographic

CHAD infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHAD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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