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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).|
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Human ckmm ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human ckmm ELISA Kit PicoKine™ (96 Tests). Quantitate Human CKM in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05mU/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ckmm ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1751)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: natural protein
0.15mU/ml - 10mU/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1751 is reactive to CKM in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
EK1751 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1751-CAP||Anti-Human CKM Pre-coated 96-well strip microplate||1|
|EK1751-ST||Human CKM Standard||2 vials, 10 mU/tube|
|EK1751-DA||Human CKM Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human ckmm PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Creatine Kinase, Muscle/CKMM, Dilution ratio of 1:20, concentration in serum is 30-50mU/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ckmm ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Creatine Kinase, Muscle/CKMM in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (mU/ml)||Lot 2 (mU/ml)||Lot 3 (mU/ml)||Lot 4 (mU/ml)||Mean (mU/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CKM (Source: Uniprot.Org, NCBI)
Creatine kinase M-type
ATP:guanido phosphotransferase family
CKM; CKMM; CKMMcreatine kinase M-type; Creatine kinase M chain; Creatine Kinase MM; creatine kinase, muscle; creatine kinase-M; EC 2.7.3; EC 188.8.131.52; M-CK CKM CKMM, CPK-M, M-CK creatine kinase, M-type creatine kinase M-type|creatine kinase M chain|creatine kinase, muscle|creatine phosphokinase M-type*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CKM, check out the CKM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CKM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1751
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