Human CLEC11A ELISA Kit PicoKine™

Human CLEC11A ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLEC11A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1943
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human CLEC11A ELISA Kit PicoKine™

See all SCGF/CLEC11a products

SKU/Catalog Number

EK1943

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CLEC11A ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLEC11A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CLEC11A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1943)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-F323

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1943 is reactive to CLEC11A in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1943 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1943-CAP Anti-Human CLEC11A Pre-coated 96-well strip microplate 1
EK1943-ST Human CLEC11A Standard 2 vials, 10 ng/tube
EK1943-DA Human CLEC11A Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0350.1360.2440.3990.6601.1291.5242.026

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SCGF/CLEC11a, Dilution ratio of 1:20, concentration in serum is 12-30ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CLEC11A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2701292473228413474986
Standard deviation19.4481.40212.9415.0586.21209.41
CV (%)7.2%6.3%4.5%5.3%6.4%4.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SCGF/CLEC11a in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127028430529628813.144.5%
Sample 21292129111471271125060.194.8%
Sample 347324272448941684415216.464.9%
*number of samples for each test n=16.

Gene/Protein Information For CLEC11A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y240

Gene ID

6320

Gene Name

CLEC11A

Full Name

C-type lectin domain family 11 member A

Weight

35.695kDa

Alternative Names

CLEC11a; CLECSF3; LSLCL; SCGF CLEC11A CLECSF3, LSLCL, P47, SCGF C-type lectin domain containing 11A C-type lectin domain family 11 member A|C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3|C-type lectin superfamily member 3|lymphocyte secreted C-type lectin|lymphocyte secreted long form of C-type lectin|osteolectin|stem cell growth factor|stem cell growth factor; lymphocyte secreted C-type lectin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLEC11A, check out the CLEC11A Infographic

CLEC11A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLEC11A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1943

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6 Customer Q&As for Human CLEC11A ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated SCGF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SCGF antigen?

A. Garcia

Verified customer

Asked: 2020-10-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-10-24

Question

Q: Can SCGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Carter

Verified customer

Asked: 2020-10-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-10-12

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-02-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-02-02

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring SCGF using the EK1943 Human SCGF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-06-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Baker

Verified customer

Asked: 2018-02-13

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-02-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-07-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-07-01

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