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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CLEC11A ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CLEC11A ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLEC11A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CLEC11A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1943)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A22-F323
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1943 is reactive to CLEC11A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1943 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SCGF/CLEC11a
This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. It is mapped to 19q13.33.
|EK1943-CAP||Anti-Human CLEC11A Pre-coated 96-well strip microplate||1|
|EK1943-ST||Human CLEC11A Standard||2 vials, 10 ng/tube|
|EK1943-DA||Human CLEC11A Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CLEC11A PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SCGF/CLEC11a, Dilution ratio of 1:20, concentration in serum is 12-30ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CLEC11A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of SCGF/CLEC11a in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CLEC11A (Source: Uniprot.Org, NCBI)
C-type lectin domain family 11 member A
CLEC11a; CLECSF3; LSLCL; SCGF CLEC11A CLECSF3, LSLCL, P47, SCGF C-type lectin domain containing 11A C-type lectin domain family 11 member A|C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3|C-type lectin superfamily member 3|lymphocyte secreted C-type lectin|lymphocyte secreted long form of C-type lectin|osteolectin|stem cell growth factor|stem cell growth factor; lymphocyte secreted C-type lectin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CLEC11A, check out the CLEC11A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CLEC11A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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