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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CNDP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CNDP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1957)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S27-H507
6.25 ng/ml - 400 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1957 is reactive to CNDP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1957 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Carnosine Dipeptidase 1/CNDP1
Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region.
|EK1957-CAP||Anti-Human CNDP1 Pre-coated 96-well strip microplate||1|
|EK1957-ST||Human CNDP1 Standard||2 vials, 200 ng/tube|
|EK1957-DA||Human CNDP1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CNDP1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Carnosine Dipeptidase 1/CNDP1, Dilution ratio of 1:60000, concentration in serum is around 320ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CNDP1/Carnosine Dipeptidase 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Carnosine Dipeptidase 1/CNDP1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CNDP1 (Source: Uniprot.Org, NCBI)
peptidase M20A family
beta-Ala-His dipeptidase; Carnosinase 1; carnosine dipeptidase 1 (metallopeptidase M20 family); Carnosine Dipeptidase 1; CN1MGC102737; CNDP dipeptidase 1; CNDP1; CPGL2; CPGL2MGC142072; EC 188.8.131.52; Glutamate carboxypeptidase-like protein 2; HsT2308; MGC10825; Serum carnosinase CNDP1 CN1, CPGL2, HsT2308 carnosine dipeptidase 1 beta-Ala-His dipeptidase|CNDP dipeptidase 1|carnosinase 1|carnosine dipeptidase 1 (metallopeptidase M20 family)|glutamate carboxypeptidase-like protein 2|serum carnosinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CNDP1, check out the CNDP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CNDP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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