Human Cortisol ELISA Kit

Cortisol ELISA kit

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Cortisol. 96 wells/kit. Cited in 3 publication(s).

Product Info Summary

SKU: EK7002
Size: 96wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum or plasma

Product Name

Human Cortisol ELISA Kit

See all Cortisol products

SKU/Catalog Number




*Question: How many samples can I assay/run in this kit?


Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Cortisol. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Cortisol ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7002)

Clonality of Antibodies

See Datasheet for details


20 ng/ml

Assay Range

20-500 ng/ml


There is no detectable cross-reactivity.

Reactive Species

EK7002 is reactive to Cortisol in Human samples

Validated Sample Types

Serum or plasma

Application Guarantee

EK7002 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Microwells coated with Cortisol MAb12x8x1 Microwells
Cortisol Standards6 vials ( ready to use) 0.5 ml
Biotin Reagent 1 bottle ( ready to use) 7 ml
Enzyme Conjugate (20x)1 vial 0.7ml
Assay Diluent1 bottle (ready to use) 12ml
TMB Substrate1 bottle (ready to use) 12ml
Stop Solution1 bottle (ready to use) 12ml
20X Wash concentrate1 bottle 25ml

Standard Concentrations and example data

OD 450 nmConc. ng/mL
Std 12.770
Std 21.4220
Std 30.7950
Std 40.43100
Std 50.22200
Std 60.12500

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster product! Applicable to all scientists worldwide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)02050100200500

Data Example Images

Gene/Protein Information For Cortisol (Source:, NCBI)

Uniprot ID

Gene ID

Gene Name


Full Name


*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cortisol, check out the Cortisol Infographic

Cortisol infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cortisol: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected]

EK7002 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The immediate and durable effects of yoga and physical fitness exercises on stress

Effect of residential proximity to the lignite-fired power plant on depression, sleep quality, and morning salivary cortisol in the elderly

The immediate and durable effects of yoga and physical fitness exercises on stress

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2 Customer Q&As for Human Cortisol ELISA Kit


Hello Support: I am using your product EK7002 for mouse samples, however the amount of the sample is relatively small. Could I possibly use EDTA or something to prevent it from clotting in order to maximize the samples?

Verified Customer

Verified customer

Asked: 2019-11-12


Our product EK7002 is validated only for use in human plasma and serum samples. Unfortunately, the product EK7002 have not been tested in mouse samples. You can use EDTA to prevent the samples from clotting and it should be centrifuged and frozen until tested.

Boster Scientific Support

Answered: 2019-11-12


Does this Human Cortisol ELISA kit recognize total or free Cortisol?

Verified Customer

Verified customer

Asked: 2019-10-21


The product Human Cortisol ELISA kit detects total cortisol.

Boster Scientific Support

Answered: 2019-10-21


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