Product Info Summary
SKU: | EK7002 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum or plasma |
Product info
Product Name
Human Cortisol ELISA Kit
SKU/Catalog Number
EK7002
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Cortisol. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Cortisol ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7002)
Clonality of Antibodies
See Datasheet for details
Sensitivity
20 ng/ml
Assay Range
20-500 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7002 is reactive to in Human samples
Validated Sample Types
Serum or plasma
Application Guarantee
EK7002 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity |
Microwells coated with Cortisol MAb | 12x8x1 Microwells |
Cortisol Standards | 6 vials ( ready to use) 0.5 ml |
Biotin Reagent | 1 bottle ( ready to use) 7 ml |
Enzyme Conjugate (20x) | 1 vial 0.7ml |
Assay Diluent | 1 bottle (ready to use) 12ml |
TMB Substrate | 1 bottle (ready to use) 12ml |
Stop Solution | 1 bottle (ready to use) 12ml |
20X Wash concentrate | 1 bottle 25ml |
Standard Concentrations and example data
OD 450 nm | Conc. ng/mL | |
---|---|---|
Std 1 | 2.77 | 0 |
Std 2 | 1.42 | 20 |
Std 3 | 0.79 | 50 |
Std 4 | 0.43 | 100 |
Std 5 | 0.22 | 200 |
Std 6 | 0.12 | 500 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (ng/ml) | 0 | 20 | 50 | 100 | 200 | 500 |
O.D. | 2.639 | 1.214 | 0.569 | 0.311 | 0.156 | 0.064 |
Data Example Images
Click image to see more details
Click image to see more details
Protein Target Info & Infographic
Gene/Protein Information For (Source: Uniprot.org, NCBI)
Gene Name
Full Name
Weight
For more info on , check out the Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Cortisol ELISA Kit (EK7002)
Hello CJ!
EK7002 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The immediate and durable effects of yoga and physical fitness exercises on stress
Effect of residential proximity to the lignite-fired power plant on depression, sleep quality, and morning salivary cortisol in the elderly
The immediate and durable effects of yoga and physical fitness exercises on stress
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Human Cortisol ELISA Kit
Question
Hello Support: I am using your product EK7002 for mouse samples, however the amount of the sample is relatively small. Could I possibly use EDTA or something to prevent it from clotting in order to maximize the samples?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Our product EK7002 is validated only for use in human plasma and serum samples. Unfortunately, the product EK7002 have not been tested in mouse samples. You can use EDTA to prevent the samples from clotting and it should be centrifuged and frozen until tested.
Boster Scientific Support
Answered: 2019-11-12
Question
Does this Human Cortisol ELISA kit recognize total or free Cortisol?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The product Human Cortisol ELISA kit detects total cortisol.
Boster Scientific Support
Answered: 2019-10-21