Human Cortisol ELISA Kit

Product Name

Human Cortisol ELISA Kit

SKU/Catalog Number

EK7002

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Cortisol. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Cortisol ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7002)

Clonality of Antibodies

See Datasheet for details

Sensitivity

20 ng/ml

Assay Range

20-500 ng/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7002 is reactive to in Human samples

Validated Sample Types

Serum or plasma

Application Guarantee

EK7002 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
Microwells coated with Cortisol MAb	12x8x1 Microwells
Cortisol Standards	6 vials ( ready to use) 0.5 ml
Biotin Reagent	1 bottle ( ready to use) 7 ml
Enzyme Conjugate (20x)	1 vial 0.7ml
Assay Diluent	1 bottle (ready to use) 12ml
TMB Substrate	1 bottle (ready to use) 12ml
Stop Solution	1 bottle (ready to use) 12ml
20X Wash concentrate	1 bottle 25ml

Standard Concentrations and example data

	OD 450 nm	Conc. ng/mL
Std 1	2.77	0
Std 2	1.42	20
Std 3	0.79	50
Std 4	0.43	100
Std 5	0.22	200
Std 6	0.12	500

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	20	50	100	200	500
O.D.	2.639	1.214	0.569	0.311	0.156	0.064

Data Example Images

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