Human CPB1 ELISA Kit PicoKine®

Carboxypeptidase B1/CPB1 ELISA kit for Human

Human CPB1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CPB1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2180
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Product Name

Human CPB1 ELISA Kit PicoKine®

View all Carboxypeptidase B1/CPB1 ELISA kits

SKU/Catalog Number

EK2180

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CPB1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CPB1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CPB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2180)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H16-Y417

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2180 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2180 is reactive to CPB1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

EK2180 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Carboxypeptidase B1/CPB1

Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.

Kit Components

Catalog Number Description Quantity
EK2180-CAP Anti-Human CPB1 Pre-coated 96-well strip microplate 1
EK2180-ST Human CPB1 Standard 2 vials, 20 ng/tube
EK2180-DA Human CPB1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)03126251,2502,5005,00010,00020,000
O.D.0.0330.0690.1440.2950.4840.9761.6522.379

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CPB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5461734726454917537636
Standard deviation34.4104.04450.3734.59103.43465.8
CV(%)6.3%6%6.2%6.3%5.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carboxypeptidase B1/CPB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 154656554255155123.694.3%
Sample 21734172617541771174680.334.6%
Sample 372347302716571397210410.975.7%
*number of samples for each test n=16.

Gene/Protein Information For CPB1 (Source: Uniprot.Org, NCBI)

Gene Name

CPB1

Full Name

Carboxypeptidase B

Weight

47.368kDa

Superfamily

peptidase M14 family

Alternative Names

carboxypeptidase B; carboxypeptidase B1 (tissue); Carboxypeptidase B1; CPB; CPB1; DKFZp779K1333; EC 3.4.17; EC 3.4.17.2; Pancreas-specific protein; PASP; PCPB; procarboxypeptidase B CPB1 CPB, PASP, PCPB carboxypeptidase B1 carboxypeptidase B|carboxypeptidase B1 (tissue)|pancreas-specific protein|pancreatic carboxypeptidase B|procarboxypeptidase B|protaminase|tissue carboxypeptidase B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CPB1, check out the CPB1 Infographic

CPB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CPB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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