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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.|
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Human CRISP3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CRISP3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human CRISP3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1284)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: N21-Y245
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1284 is reactive to CRISP3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
EK1284 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1284-CAP||Anti-Human CRISP3 Pre-coated 96-well strip microplate||1|
|EK1284-ST||Human CRISP3 Standard||2 vials, 100 ng/tube|
|EK1284-DA||Human CRISP3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CRISP3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CRISP-3, Dilution ratio of 1:100, concentration in serum is 10-20ng/ml.
Some articles we found to cite concentrations of CRISP-3 in samples: 15867000 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRISP3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CRISP-3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CRISP3 (Source: Uniprot.Org, NCBI)
Cysteine-rich secretory protein 3
Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa CRISP3 Aeg2, CRISP-3, CRS3, SGP28, dJ442L6.3 cysteine rich secretory protein 3 cysteine-rich secretory protein 3|specific granule protein (28 kDa)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CRISP3, check out the CRISP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRISP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1284
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