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Product Info Summary
| SKU: | EK1284 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human CRISP3 ELISA Kit PicoKine®
SKU/Catalog Number
EK1284
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CRISP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRISP3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1284)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: N21-Y245
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1284 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1284 is reactive to CRISP3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK1284 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CRISP3
Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1284-CAP | Anti-Human CRISP3 Pre-coated 96-well strip microplate | 1 |
| EK1284-ST | Human CRISP3 Standard | 2 vials, 100 ng/tube |
| EK1284-DA | Human CRISP3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.001 | 0.085 | 0.167 | 0.356 | 0.711 | 1.083 | 1.773 | 2.149 |
Data Example Images
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Human CRISP3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CRISP3, Dilution ratio of 1:100, concentration in serum and plasma is 10-20 ng/ml..
Some articles we found to cite concentrations of CRISP3 in samples: 15867000 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRISP3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 110 | 383 | 1477 | 119 | 408 | 1764 |
| Standard deviation | 4.51 | 23.75 | 66.47 | 5.59 | 26.11 | 95.26 |
| CV (%) | 4.1% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Specific Publications For Human CRISP3 ELISA Kit PicoKine® (EK1284)
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Customer Reviews
0 Reviews For Human CRISP3 ELISA Kit PicoKine®
Customer Q&As
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Find answers in Q&As, reviews.
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12 Customer Q&As for Human CRISP3 ELISA Kit PicoKine®
Question
Q: Can CRISP-3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-10-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-02
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Martin
Verified customer
Asked: 2020-01-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-19
Question
Could you help with this inquiry regarding Human CRISP3 PicoKine
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
EK1284 is only for freely circulating CRISP-3.
Boster Scientific Support
Answered: 2019-11-26
Question
What is the suggested dilution ratio for serum samples using EK1284?
Verified customer
Asked: 2019-11-04
Answer
The suggested dilution ratio for serum samples using the Human CRISP3 ELISA Kit PicoKine™ (EK1284) is 1:100.
Boster Scientific Support
Answered: 2019-11-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-19
Question
CRISP-3 is usually found in a complex bound with A1B- Glycoprotein. Will EK1284 detect CRISP-3 when bound to a glycoprotein or will it only detect freely circulating CRISP-3?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
EK1284 will only detect freely circulating CRISP-3.
Boster Scientific Support
Answered: 2018-07-26
Question
Q: how to proceed with the analysis of ELISA data? I have performed CRISP-3 level in urine.
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-07-24
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CRISP-3 using the EK1284 Human CRISP-3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-06-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Mehta
Verified customer
Asked: 2017-05-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-02
Question
Q: can I use citrate plasma as samples in Human CRISP-3 Picokine® ELISA Kit (Catalog # EK1284)?
Verified Customer
Verified customer
Asked: 2016-06-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CRISP-3 causing degradation of its protein structure. CRISP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CRISP-3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-06-07
Question
Q: how can I thaw whole blood sample for CRISP-3 ELISA after freezing?
V. Edwards
Verified customer
Asked: 2015-09-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CRISP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-09-24
Question
Q: if the enzyme conjugated CRISP-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CRISP-3 antigen?
Verified Customer
Verified customer
Asked: 2014-08-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-08-17
