Human CRP/C Reactive Protein ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CRP. 96wells/kit, with removable strips. Cited in 5 publication(s).
Human CRP/C Reactive Protein ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human CRP/C Reactive Protein ELISA Kit PicoKine™
See all CRP primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human C-Reactive Protein/CRP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. C-Reactive Protein/CRP tissue specificity: Found in plasma.|
|Cite This Product||Human CRP/C Reactive Protein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1316)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen||Expression system for standard: NSO; Immunogen sequence: F17-P224|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||C-reactive protein|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Human CRP/C Reactive Protein ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1316-CAP||96-well plate precoated with anti-Human CRP antibody||1|
|EK1316-ST||lyophilized recombinant Human CRP standard||20ng/tube|
|EK1316-DA||biotinylated anti-Human CRP antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1316 Human CRP/C Reactive Protein ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human CRP/C Reactive Protein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human CRP/C Reactive Protein ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 5
|Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...||PubMed ID 22509346|
|Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.||PubMed ID 24137221|
|Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.||PubMed ID 24575134|
|Liu B, Piao X, Guo L, Wang G, Sun W, Gao L, Zheng X, Fang Y. Evid Based Complement Alternat Med. 2017;2017:7323129. doi: 10.1155/2017/7323129. Epub 2017 Nov 20. A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylat...||PubMed ID 29358969|
|Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals||PubMed ID 29114515|
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Note: sample size takes longer to process, average 5-7 days.
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