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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human CRP/C Reactive Protein ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CRP/C Reactive Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human CRP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRP/C Reactive Protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1316)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: F17-P224
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1316 is reactive to CRP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1316 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C-Reactive Protein/CRP
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
|EK1316-CAP||Anti-Human CRP Pre-coated 96-well strip microplate||1|
|EK1316-ST||Human CRP Standard||2 vials, 20 ng/tube|
|EK1316-DA||Human CRP Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CRP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect C-Reactive Protein/CRP, Dilution ratio of 1:5000, concentration in serum is around 10ug/ml.
Some articles we found to cite concentrations of C-Reactive Protein/CRP in samples: 25785170, 25722922, 18775894, 21576827 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRP/C Reactive Protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of C-Reactive Protein/CRP in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CRP (Source: Uniprot.Org, NCBI)
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CRP, check out the CRP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1316 has been cited in 15 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Research on the correlation between the fibrinogen β and attack of pediatric pneumonia.
A new scoring system for early diagnosis of ventilator-associated pneumonia: LUPPIS
Role of Different Blood Purification Nursing Models in Uremic Patients: A Preliminary Report
Effect of Ambient PM2.5-Bound BbFA and DahA on Small Airway Dysfunction of Primary Schoolchildren in Northeast China
Soy milk consumption, markers of inflammation and oxidative stress in women with rheumatoid arthritis: A randomised cross‐over clinical trial
Altered gut microbiota after traumatic splenectomy is associated with endotoxemia
Effect of preoperative inhaled budesonide on pulmonary injury after cardiopulmonary bypass: A randomized pilot study
Effects of dancing compared to walking on cardiovascular risk and functional capacity of older women: A randomized controlled trial
Low-Fat, High-Fiber Diet Reduces Markers of Inflammation and Dysbiosis and Improves Quality of Life in Patients With Ulcerative Colitis
Association between C‐reactive protein gene variant and treatment efficacy of etanercept in ankylosing spondylitis patients receiving hip arthroplasty
Koh Y, Park KS. J Exerc Rehabil. 2017 Aug 29;13(4):472-476. doi: 10.12965/jer.1735066.533. eCollection 2017 Aug. Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals
Liu B, Piao X, Guo L, Wang G, Sun W, Gao L, Zheng X, Fang Y. Evid Based Complement Alternat Med. 2017;2017:7323129. doi: 10.1155/2017/7323129. Epub 2017 Nov 20. A New Chinese Medicine Intestine Formula Greatly Improves the Effect of Aminosalicylat...
Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.
Shi L, Song J, Zhang X, Li Y, Li H. Exp Ther Med. 2013 Aug;6(2):532-536. Epub 2013 May 28. Correlation Between The Microinflammatory State And Left Ventricular Structural And Functional Changes In Maintenance Haemodialysis Patients.
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
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