Human DCBLD2 ELISA Kit PicoKine®

DCBLD2/ESDN ELISA kit for Human

Human DCBLD2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DCBLD2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2183
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human DCBLD2 ELISA Kit PicoKine®

View all DCBLD2/ESDN ELISA kits

SKU/Catalog Number

EK2183

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human DCBLD2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human DCBLD2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human DCBLD2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2183)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q67-A528

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2183 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2183 is reactive to Dcbld2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2183 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of DCBLD2/ESDN

Discoidin, CUB and LCCL domain-containing protein 2 is a protein that in humans is encoded by the DCBLD2 gene. Involved in negative regulation of cell growth and wound healing. Located in cell surface. Is integral component of plasma membrane.

Kit Components

Catalog Number Description Quantity
EK2183-CAP Anti-Human Dcbld2 Pre-coated 96-well strip microplate 1
EK2183-ST Human Dcbld2 Standard 2 vials, 10 ng/tube
EK2183-DA Human Dcbld2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0280.0810.1470.2790.5091.0861.3252.261

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human DCBLD2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11443512161204411268
Standard deviation5.0218.7147.427.6828.6773.54
CV(%)4.4%4.3%3.9%6.4%6.5%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DCBLD2/ESDN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11141099812311154.5%
Sample 243544145246244822.825.1%
Sample 31216125112631196123268.965.6%
*number of samples for each test n=16.

Gene/Protein Information For Dcbld2 (Source: Uniprot.Org, NCBI)

Gene Name

Dcbld2

Full Name

Discoidin, CUB and LCCL domain-containing protein 2

Weight

83.774kDa

Alternative Names

CLCP1; CLCP11700055P21Rik; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; DCBLD2; discoidin, CUB and LCCL domain containing 2; discoidin, CUB and LCCL domain-containing protein 2; Endothelial and smooth muscle cell-derived neuropilin-like protein; ESDN; ESDNcoagulation factor V/VIII-homology domains protein 1 Dcbld2|1700055P21Rik, AW146002, CLC, CLCP1, Es, Esdn|discoidin, CUB and LCCL domain containing 2|discoidin, CUB and LCCL domain-containing protein 2|endothelial and smooth muscle cell-derived neuropilin-like protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Dcbld2, check out the Dcbld2 Infographic

Dcbld2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Dcbld2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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